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In the present study 3,800 buffaloes were screened for skin depigmentation irrespective of age, sex and breed in which 18 were found leukodermic. The cases were diagnosed on the basis of history and clinical signs. Hematology and serum micro minerals status were evaluated. In the present study a non significant change in value of Hb, PCV, TEC, TLC, MCV, MCH, and MCHC in leukodermic buffaloes were obtained. The mean activities of ALT, AST, and ALP were significantly higher in Leucodermic buffaloes as compared to healthy buffaloes. A significant decline in serum copper and zinc levels from 77.69+1.39 and 88.38+1.13 to 52.37+0.59 and 73.65+0.88 respectively in Leucodermic buffaloes were noticed.