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Anestrus is a functional disorder of reproductive cycle in cattle and buffalo which is characterized by absence of overt signs of estrus and also affecting the livestock enterprise to a great extent. Incidence of anestrus is more in buffalo than the cattle, and problem is severe during summer. The present study was carried out to see the efficacy of lugol’s iodine for the initiation of ovarian cyclicity in postpartum true anestrus buffaloes. Confirmation of true anestrus in 20 buffaloes was done by finding smooth ovaries at rectal examination, out of 20, 10 buffaloes was treated with Lugol’s iodine (1:50) ratio 30 ml I/U once only whereas, the remaining 10 buffaloes were serve as control for treated group and no treatment was given to such animals. The result for induction of estrus was 70% (7/10) and the conception rate was 85.71% (6/7). Lugol’s iodine treatment is cheaper and effective means of management of anestrus but response has been variable.