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The present study was carried out to examine the effect of GnRH and PGF2 α administration during early post partum period (PP) on ovarian activities and initiation of PP estrus in Murrah buffaloes. A total of thirty six Murrah buffaloes were selected and divided equally into three groups comprising twelve buffaloes each. Buffaloes in Group I were i.m. injected with GnRH as busreline acetate ( 10 µg) while those in group II were i.m. injected with PGF2 α as tiaprosttrometamol (0.750 mg) on day 14 PP. The buffaloes in Group III were kept as control. The experimental buffaloes were examined with 7.5 transrectal probe on day 14, 21 and 28 PP. On day 14 PP, 14 (38.88%) ovaries showed small follicular development and 4 (11.11%) ones showed multiple follicular activity when observed ultrasonographically. On day 21 postpartum, 4 (11.11%) ovaries showed small follicular development, 11 (30.50%) ovaries showed multiple follicular activity and 18 (50.00%) ones indicated good follicular development when observed ultrsonographically. On day 28 PP, 3 (9.37%) ovaries showed small follicular development and 3 (9.37%) ones showed multiple follicular activities whereas 26 (81.25%) ones indicated good follicular development when observed ultrasonographically. The post partum ovarian activity was initiated with average 3.5±0.150, 3.25±0.130 and 4±0.467 weeks in Group I, II and III, respectively. From the present study it can be concluded that PGF2 a administration during early PP hastens the initiation of post-partum estrus in buffaloes and transrectal ultrasonography is good tool for monitoring the slight follicular developments.