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The present research experiment was carried out to evaluate the effect of GnRH and PGF2α administration on post partum fertility in retained placenta buffaloes. Total thirty six Murrah buffaloes with retained placenta condition were selected and the placentas were removed manually and intra-uterine boluses were kept (3 boluses per day for 3 consecutive days post-partum) in experimental buffaloes. The buffaloes were divided into three groups comprising twelve buffaloes in each group. Buffaloes from Group-I were treated with injection buserlin acetate (10 µg) i/m while from Group-II with injection tiaprost-trometamol 0.750 mg i/m on day 14 post calving while buffaloes from Group-III were kept as control. The average days required for involution of uterus were 29.16±0.78, 27.41±0.58 and 31.5±1.05 while average days required for exhibition of first postpartum oestrus were 49.33±5.86, 47.66±4.18 and 67.08±4.42 in Group-I, II and III, respectively. The first service conception rate was highest (33.33%) in Group-II while 25 and 16% in Group-I and III, respectively.