β(1,4)-Galactosyltransferase-I gene polymorphisms in Pakistani Nili Ravi buffalo

Authors

  • Aamir Sohail Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore, Pakistan
  • Asif Nadeem Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore, Pakistan
  • Masroor Ellahi Babar Virtual University of Pakistan, Lahore, Pakistan
  • Tanveer Hussain Virtual University of Pakistan, Lahore, Pakistan
  • Akhtar Ali Virtual University of Pakistan, Lahore, Pakistan
  • Wasim Shehzad Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore, Pakistan
  • Maryam Javed Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore, Pakistan

Keywords:

Buffaloes, Bubalus bubalis, Polymorphisms, Gene, β4GalT-I Gene, α-lactalbumin, Nili Ravi, Biological function, Develop relations of diversity, Pakistan

Abstract

β4GalT-I interacts with α-lactalbumin to form the lactose synthase in mammary gland. Considering the biological function of the lactose synthase complex, β4GalT-I gene can be considered as a candidate gene for milk production in dairy animals. The bovine β4GalT-I gene, 3,283 base pairs in length, is mapped on chromosome 8. The present research work was planned to identify the polymorphism in β4GalT-I gene in Nili Ravi buffalo. Blood Samples were collected, DNA was extracted and primers were designed for PCR. After amplification, PCR products were sequenced for the identification of allelic variation. Thirteen polymorphic, six in coding and seven in noncoding region of gene, were identified. This is a first report toward genetic screening of β4GalT-I gene at molecular level in Nili Ravi buffalo. The present study will provide a better selection to develop association of identified polymorphisms with production traits in buffalo population.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2016-09-30

How to Cite

Sohail, A., Nadeem, A., Babar, M. E., Hussain, T., Ali, A., Shehzad, W., & Javed, M. (2016). β(1,4)-Galactosyltransferase-I gene polymorphisms in Pakistani Nili Ravi buffalo. Buffalo Bulletin, 35(3), 399–403. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/1116

Issue

Section

Original Article

Most read articles by the same author(s)