A novel SNP (c.258+43C>T) in LPL gene and association with milk production in buffaloes

Authors

  • Akhtar Ali Faculty of Science and Technology, Virtual University of Lahore, Pakistan
  • Zukhruf Baig Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Masroor Ellahi Babar Faculty of Science and Technology, Virtual University of Pakistan, Lahore, Pakistan
  • Asif Nadeem Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Tanveer Hussain Faculty of Science and Technology, Virtual University of Pakistan, Lahore, Pakistan
  • Rashid Saif Faculty of Science and Technology, Virtual University of Pakistan, Lahore, Pakistan
  • Maryam Javed Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan

Keywords:

Bubalus bubalis, Polymorphism, buffaloes, Genetic Diversity, LPL gene, Azakheli, Nili-Ravi, polymorphism, genetic diversity

Abstract

Buffalo milk is the most preferred commodity in Pakistan due to high fat contents. Hydrolysis of circulating triglycerides and uptake of fatty acids in the mammary gland is regulated by lipoprotein lipase (LPL). T his study is designed to identify single nucleotide polymorphisms (SNPs) in LPL gene among high and low milk producing buffalo breeds of Pakistan. We selected samples (n=50) of each Nili-Ravi a high milk producing and Azakheli a low milk producing buffalo breeds. Blood samples were collected for DNA extraction. LPL region of exon 2 region along with exon/intron boundaries were sequenced and data was analyzed for variation detection. Allele frequency was calculated using Hardy-Weinberg equation and in-silico analysis was performed for functional prediction and genetic diversity assessment. We found one single nucleotide polymorphism c.258+43C>T in the intronic region of LPL gene. This polymorphism followed the Hardy-Weinberg equilibrium in both Nili-Ravi (P-value = 0.538) and Azakheli (P-value = 0.077). Association of T-allele analysis predicted it significantly higher (P-value = 0.009) in Nili-Ravi. We found one novel SNP (c.258+43C>T) in the LPL gene in both high and low milk producing buffalo breeds of Pakistan but high in Nili-Ravi. Lower splice site effect suggests its less strength of association with milk producing trait.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2019-06-29

How to Cite

Ali, A., Baig, Z., Babar, M. E., Nadeem, A., Hussain, T., Saif, R., & Javed, M. (2019). A novel SNP (c.258+43C>T) in LPL gene and association with milk production in buffaloes. Buffalo Bulletin, 38(2), 327–333. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/437

Issue

Section

Original Article

Most read articles by the same author(s)