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Nutritional deficiency including mineral deficiency may decrease reproductive efficiency in buffaloes. Therefore, aim of the present study was to assess effect of minerals Calcium (Ca) Magnesium (Mg) and inorganic phosphorus (Pi) on cyclicity of Nili Ravi Buffaloes. The present experiment was performed at Livestock Experiment Station, Bhunikey. The female buffaloes (n=90) were divided into 3 groups; cyclic (n=30), non-cyclic (n=30) and repeat breeders (n=30). Mineral profile was measured through serum of the buffaloes under study. Calcium and Magnesium were measured through calorimetric method while inorganic phosphorus was measured through UV method. The level of calcium was significantly (P<0.05) higher in normal cyclic buffaloes than that of the non-cyclic and repeat breeders. The level of magnesium was non-significant in normal cyclic and non-cyclic buffaloes, while it was significantly (P<0.05) higher in repeat breeders as compared to cyclic and non-cyclic buffaloes. Phosphorus was not in balance in non-cyclic and repeat breeders. It is concluded from the present study that Ca: P should be 2:1.