Molecular characterization of buffalo αs1-casein gene
DOI:
https://doi.org/10.56825/bufbu.2022.4131234Keywords:
Bubalus bubalis, buffaloes, alpha s1-Casein, mRNA sequence, amino acid sequenceAbstract
The caseins (Alpha s1, Alpha s2, beta and kappa) comprise the major protein component of ruminant milk and are secreted in the form of stable calcium phosphate micelles. The present study was proposed to characterize αs1-casein gene in buffalo at the molecular level to determine complete αs1-casein cDNA sequence. Sequencing results were analyzed and aligned with available mRNA GenBank sequences of other domestic animals (Accession AAB34797, NM_001009795, X72221, AY344966, NM_181029, AY948385 and BC109618). The complete amino acid coding region of the buffalo αs1- casein was deduced from cloned cDNA generated from mammary tissue mRNA. Amino acid sequence comprises of 199 amino acid residues. When aligned with previously reported buffalo sequence (Accession-AY948385) it showed one amino acid (Glutamine) deletion at amino acid position 78.
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References
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Farrell, H.M. Jr., R. Jimenez-Flores, G.T. Bleck, E.M. Brown, J.E. Butler, L.K. Creamer, C.L. Hicks, C.M. Hollar, K.F. Ng-Kwai-Hang and H.E. Swaisgood. 2004. Nomenclature of the proteins of cows’ milk-sixth revision. J. Dairy Sci., 87(6): 1641-1674. DOI: 10.3168/jds.S0022-0302(04)73319-6
Ferranti, P., F. Addeo, A. Malorni, L. Chianese, C. Leroux and P. Martin. 1997. Differential splicing of pre-messenger RNA produces multiple forms of mature caprine alpha(s1)-casein. Eur. J. Biochem., 249(1): 1-7. DOI: 10.1111/j.1432-1033.1997.t01-5-00001.x
Ferranti, P., S. Lilla, L. Chianese and F. Addeo. 1999. Alternative nonallelic deletion is constitutive of ruminant αs1-casein. J. Protein Chem., 18: 595-602. DOI: 10.1023/a:1020659518748
Ferranti, P., A. Malorni, G. Nitti, P. Laezza, R. Pizzano, L. Chianese and F. Addeo. 1995. Primary structure of ovine αs1-casein: Localization of phosphorylation sites and characterization of genetic variants variants A, C and D. J. Dairy Res., 62: 281-296. DOI: 10.1017/S0022029900030983
Goldamer, T., R. Weikard, M.N. Miziara, R.M. Brunner, R. Agrawala, A.A. Schaffer, J.E. Womack and M.E.J. Amaral. 2007. A radiation hybrid map of river buffalo (Bubalus bubalis) chromosome 7 and comparative mapping to the cattle and human genomes. Cytogenet Genome Res., 119(3-4): 235-241. DOI: 10.1159/000112067
Hall, T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. S., 41: 95-98.
Koczan, D., G. Hobom and H.M. Seyfert. 1991. Genomic organization of the bovine alpha-S1 casein gene: Nucl. Acids. S., 19(20): 5591-5596. DOI: 10.1093/nar/19.20.5591
Larkin, M.A., G. Blackshields, N.P. Brown, R. Chenna, P.A. McGettigan, H. McWilliam, F. Valentin, I.M. Wallace, A. Wilm, R. Lopez, J.D. Thompson, T.J. Gibson and D.G. Higgins. 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23(21): 2947-2948. DOI: 10.1093/bioinformatics/btm404a
Ng-kwai-Hang, K.F., J.F. Hayes, J.E. Moxley and H.G. Monardes. 1987. Variation in milk protein concentrations associated with genetic polymorphism and environmental factors. J. Dairy Sci., 70(3): 563-570. DOI: 10.3168/jds.S0022-0302(87)80042-5
Nicholas, K.B. and H.B. Nicholas Jr. 1997. GeneDoc: A Tool for Editing and Annotating Multiple Sequence Alignments. Available on: https://www.psc.edu/biomed/genedoc/
Rambaut, A. 2008. FigTree. Institute of Evolutionary Biology, University of Edinburgh, Edinburgh. Available on: http://tree.bio.ed.ac.uk/software/figtree
Rijnkels, M. 2002. Multispecies comparison of the casein gene loci and evolution of casein gene family. J. Mammary. Gland Biol., 7(3): 327-345. DOI: 10.1023/a:1022808918013
Sambrook, J. and D. Russell. 2001. Molecular Cloning. A Laboratory Manual, 3rd ed. Cold Spring Harbor Press, New York, USA.
Sang, B.C., B.S. Ahn, B.D. Sang, Y.Y. Cho and A. Djajanegara. 1994. Association of genetic variants of milk proteins with lactation traits in Holstein cows, p. 217-211. In 7th Asian Australasian Animal Production Animal Science Congress Proceedings, Bali, Indonesia.
Sukla, S., T.K. Bhattacharya, R.T. Venkatachalapathy, P. Kumar and A. Sharma. 2007. Molecular cloning and characterization of buffalo αs1-casein gene. DNA Seq., 18(5): 334-340. DOI: 10.1080/10425170701388974
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Published
2022-09-28
How to Cite
Pramanik, B. K., Batabyal, S., Maity, A., De, S., Chattopadhyay, S., & Barui, A. (2022). Molecular characterization of buffalo αs1-casein gene. Buffalo Bulletin, 41(3), 447–454. https://doi.org/10.56825/bufbu.2022.4131234
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