Immunochemical characterization of purified buffalo beta-lactoglobulin

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Ranjit Aich Subhasis Batabyal Siddhartha Narayan Joardar


Beta-lactoglobulin (β-lg) is a major whey protein found in cow milk and other ruminants, deer, bison, and buffalo. β-lg is considered as one of the main allergenic components in bovine milk and therefore the modification of β-lg is considered as a promising treatment for milk allergy. Thus the present study was conducted to assess the antigenicity of purified β-lg that can be exploited for detection of buffalo milk-protein intolerance. β-lg was purified from milk of Murrah buffalo of Haringhata Farm, West Bengal (India) by combination of gel filtration (using Sephacryl S-200) and anion-exchange chromatography (using DEAE-Sepharose). The molecular weight was derived as 18.05 kDa in 15 percent one-dimensional SDS-PAGE. Antigenicity (seroreactivity) of purified β-lg was evaluated by double immuno diffusion (DID), western blotting, dipstick ELISA and indirect ELISA using hyperimmune sera raised in rabbit against purified buffalo β-lg. In indirect ELISA, titer of rabbit serum against buffalo β-lg was observed to be in between 200-400. Therefore, the study revealed that purified buffalo β-lg is highly antigenic and a seroreactive protein and may be used for detection of buffalo milk proteinintolerance using rapid, user-friendly, cost effective sero-diagnostic technique/tool

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AICH, Ranjit; BATABYAL, Subhasis; JOARDAR, Siddhartha Narayan. Immunochemical characterization of purified buffalo beta-lactoglobulin. Buffalo Bulletin, [S.l.], v. 35, n. 2, p. 269-275, june 2016. ISSN 2539-5696. Available at: <>. Date accessed: 28 sep. 2021.
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