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Present study deals with efficacy of AV/FRC/18 (Keetguard Liquid), a Polyherbal ectoparasiticidal against lice and tick infestation in buffaloes. All the buffaloes were infested with Haematopinus spp. (Lice) and Boophilus spp. (Ticks). Lice infestation was mostly seen on scapular region, back, groin and axilla region while tick Infestation was mostly seen in the region of perineum, inner thigh, ears, neck, groin and axilla regions. Percent efficacy of AV/FRC/18 against lice and tick infestation was recorded and it was found that thrice a week application of 1:20 dilution results in 99% and 95% respectively on day 7th post-treatment and maximum period of effectiveness was found 95% and 90% respectively up to 30 days post treatment.