Role of progesterone supplementation in estrus induction in Murrah buffaloes under field conditions in non-breeding season

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Karan Sharma Jagat Bir Phogat Anand Kumar Pandey Aman Parkash Dhaka Shobna Singh Sonu Ghadwal

Abstract

The objective of present study was to assess the effect of Modified Co-synch protocol with or without progesterone device on estrus induction and conception rate in Murrah buffaloes under field conditions during summer season. The study was conducted on 30 postpartum anestrous Murrah buffaloes, divided into two groups containing 15 animals in each group. All animals were of parity between 2nd to 5th and body condition score of more than 3. Group 1 animals were treated with Modified Co-synch protocol. In Group 2 animals, in addition to Modified Co-synch protocol progesterone device was supplemented exogenously and kept in situ for 7 days. Another objective of study was to estimate plasma progesterone concentration on different days of protocols in both the groups. In Group 1, out of 15 animals that received Modified Co-synch protocol treatment, 12 (80%) animal responded and exhibited signs of heat after the prostaglandin administration and out of which four animal (33.33%) exhibited excellent symptoms, five animals (41.7%) showed medium category estrus and only three animal (25%) responded with poor estrus symptoms. Similarly in Group 2, all 15 animals that received Modified Co-synch protocol along with progesterone device for 7 days responded to treatment and expressed sign of heat and out of which seven buffaloes (46.7%) responded with excellent symptoms, five animals (33.3 %) showed medium estrus symptoms and only three animals (20%) expressed poor estrus signs. In Group 1, out of 12 animals which were inseminated, five animals (33.3%) were found to be pregnant after FTAI at induced estrus and three animals (30%) got pregnant following AI during second estrus. Thus, an overall conception rate (53.53%) with pregnancy of eight buffaloes following induced and subsequent estrous was achieved in this Group. Similarly in Group 2, out of 15 animals, seven buffaloes (46.6%) were found to be pregnant following induced estrous and three animals (37.5%) got pregnant following AI at subsequent estrous. Thus, an overall conception rate (66.67%) with pregnancy of ten buffaloes following induced and subsequent estrous was recorded in this group. Concentration of progesterone in Group 1 was 0.24±0.04, 1.38±0.11, 0.31±0.05, 0.25±0.04 ng/ml (on Day 0, 7, 9, 10) and in Group 2 was 0.26±0.04, 3.75±0.40, 0.24±0.05, 0.25±0.05 ng/ml (on Day 0, 7, 9, 10). Therefore, it was concluded that progesterone supplementation in Modified Co-synch protocol treatment during non-breeding season results in very good induction and acceptable conception rate in anestrous buffaloes under field conditions.

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SHARMA, Karan et al. Role of progesterone supplementation in estrus induction in Murrah buffaloes under field conditions in non-breeding season. Buffalo Bulletin, [S.l.], v. 40, n. 1, p. 99-106, mar. 2021. ISSN 2539-5696. Available at: <https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/2667>. Date accessed: 26 oct. 2021.
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Original Article

References

Baruselli, P.S., V.H. Barnabe, R.C. Barnabe, J.A. Visintin, J.R. Molero-Filho and R. Porto. 2001. Effect of body condition score at calving on postpartum reproductive performance in buffalo. Buffalo J., 17: 53-65.
Bhoraniya, H.L., J. Arjunbhai, A.J. Dhami, M. Naikoo, B.C. Parmar and N.P. Sarvaiya. 2012. Effect of estrus synchronization protocols on plasma progesterone profile and fertility in postpartum anoestrous Kankrej cows, Trop. Anim. Health Pro., 44(6): 1191-1197. DOI: 10.1007/s11250-011-0057-1
Biradar, S., M.K. Tandle, M.D. Suranagi, S.M. Ustrurge, N.A. Patil and Y.H. Babu. 2016. Study on efficacy of cosynch and ovsynch protocols on fertility in repeat breeder buffaloes. Buffalo Bull., 35: 737-743. Available on: https://ibic.lib.ku.ac.th/e-bulletin/IBBU201604035.pdf
Bisen, A., S.N. Shukla, M.K. Shukla and A. Mishra. 2018. Fertility response using Timed Insemination protocol in sub-oestrus buffaloes. J. Anim. Res., 8(3): 417-421. DOI: 10.13140/RG.2.2.10984.42242
Buhecha, K.V., A.J. Dhami, K.K. Hadiya, M.D. Patel, S.C. Parmasr and A. Killedar. 2015. Influence of different estrus synchronization protocols on fertility and plasma progesterone in anoestrus crossbred cows. Indian J. Anim. Reprod., 36(2): 197-201.
Buhecha, K.V., A.J. Dhami, M.D. Patel, K.K. Hadiya, R.G. Shah and V.B. Kharadi. 2016. Study on different estrus induction protocols with respect to fertility and plasma progesterone profile in anoestrus buffaloes. Indian J. Dairy Sci., 69(2): 81-85.
Busch, D.C., D.J. Schafer, D.J. Wilson, D.A. Mallory, N.R. Leitman, J.K. Haden, M.R. Ellersieck, M. F. Smith and D.J. Patterson. 2008. Timing of artificial insemination in postpartum beef cows following administration of the CO-Synch+ controlled internal drug-release protocol. J. Anim. Sci., 86(7): 1519-1525. DOI: 10.2527/jas.2008-0925
Carvalho, N.A.T., M.V. Carvalho, J.A. Visintin, F.S. Vannucci, M.F. Sá Filho, M. Nichi, R.H. Reichert and P.S. Baruselli. 2004. Uso de dispositivos intravagina is de progesterone associados ao hCG ou GnRH para sincronização da ovulação em búfalas na estação reprodutiva desfavorável. In Congresso Brasileiro de Reprodução Animal, 16, Goiânia, GO. Anais: Resumos, Brazil.
De Rensis, F., G. Ronci, P. Guarneri, B.X. Nguyen and H.G. Presicce. 2005. Conception rate after fixed time insemination following Ovsynch protocol with and without progesterone supplementation in cyclic and non-cyclic Mediterranean Italian buffaloes (Bubalus bubalis). Theriogenology, 63(7): 1824-1831. DOI: 10.1016/j.theriogenology.2004.07.024
Ghuman, S.P.S., M. Honparkhe, J. Singh and V.K. Gandotra. 2012. Ovsynch plus CIDR based fixed-time AI protocol as a the rapeutic strategy in repeat-breeder crossbred dairy cattle. Indian J. Anim. Sci., 81(3): 257-259.
Ghuman, S., M. Honparkhe and J. Singh. 2014. Comparison of ovsynch and progesterone-based protocol for induction of synchronized ovulation and conception rate in sub estrous buffalo during low-breeding season. Iran. J. Vet. Res., 15(4): 375-378.
Kumar, L., J.B. Phogat, A.K. Pandey, S.K. Phulia, S. Kumar and J. Dalal. 2016. Estrus induction and fertility response following different treatment protocols in Murrah buffaloes under field conditions. Vet. World, 9(12): 1466-1470. DOI: 10.14202/vetworld.2016.1466-1470
Lamb, G.C., J.S. Stevenson, D.J. Kesler, H.A. Garverick, D.R. Brown and B.E. Salfen. 2001. Inclusion of an intravaginal progesterone insert plus GnRH and prostaglandin F2α for ovulation control in postpartum suckled beef cows. J. Anim. Sci., 79(9): 2253-2259. DOI: 10.2527/2001.7992253x
Lima, F.S., H. Ayres, M.G. Favoreto, R.S. Bisinotto, L.F. Greco, E.S. Ribeiro, P.S. Baruselli, C.A. Risco, W.W. Thatcher and J.E.P. Santos. 2011. Effects of gonadotropin-releasing hormone at initiation of the 5-d timed artificial insemination (AI) program and timing of induction of ovulation relative to AI on ovarian dynamics and fertility of dairy. J. Dairy Sci., 94(10): 4997-5004. DOI: 10.3168/jds.2011-4240
McDougall, S., C.R. Burke, K.L. Macmillan and N.B. Williamson. 1992. The effect of pretreatment with progesterone onthe oestrous response to oestradiol-17β benzoate in the postpartum dairy cow. Proc. New. Zeal. Soc. An., 52: 157-160.
Nanda, A.S., P.S. Brar and S. Prabhakar. 2003. Enhancing reproductive performance in dairy buffalo, major constraints and achievements. Reproduction, 61: 27-36. DOI: 10.1530/biosciprocs.5.003
Nakrani, B.B., M.T. Panchal, A.J. Dhami, K.K. Hadiya, J.A. Patel and R.K. Gosai. 2014. Effect of controlled breeding techniques on fertility and plasma profile of biochemical and mineral constituents in anoestrus buffaloes. Scholars Journal of Agriculture and Veterinary Sciences, 1(4B): 299-304. Available on: http://saspjournals.com/wp-content/uploads/2014/08/SJAVS-14B299-304.pdf
Patel, K.R., A.J. Dhami, K.K. Hadiya, F.P. Savalia and N.P. Sarvaiya. 2013. Effect of CIDR and Ovsynch protocols on estrus response, fertility and plasma progesterone and biochemical profile in true anestrous crossbred cows. Indian Journal of Animal Production and Management, 29(3-4): 50-58.
Pinheiro, O.L., C.M. Barros, R.A. Figueiredo, E.R. DoValle, R.O. Encarnacao and C.R. Padovani. 1998. Estrous behavior and the estrus-to-ovulation interval in Nelore cattle (Bos indicus) with natural estrus or estrus induced with prostaglandin F2α or norgestomet and estradiol valerate. Theriogenology, 49(3): 667-681. DOI: 10.1016/S0093-691X(98)00017-X
Rhodes, F.M., C.R. Burke, B.A. Clark, M.L. Day and K.L. Macmillan. 2002. Effect of treatment with progesterone andoestradiol benzoate on ovarian follicular turnover in postpartum anoestrous cows and cows which have resumedoestrous cycles. Anim. Reprod. Sci., 69(3-4): 139-150. DOI: 10.1016/s0378-4320(01)00141-5
Thakor, D. and D. Patel. 2013. Incidence of Infertility Problems in Cattle and Buffaloes, Available on: https://en.engormix.com/dairy-cattle/articles/incidence-infertility-problems-cattle-t35803.htm
Tiwari, V.P., R.P. Tiwari, S.P. Tiwari, J. R. Khan, G.K. Mishra and M.R. Poyam. 2014. Evaluation of insulin and dextrose pretreatment to ovsynch protocol on induction of cyclicity and conception in buffaloes during summer. Haryana Veterinarian, 53(2): 89-91. Available on: https://www.luvas.edu.in/haryana-veterinarian/download/harvet-Dec2014/3.pdf
Zicarelli, L. 1997. Reproductive seasonality in buffalo. In Proceedings, 3rd Course on Biotechnology of Reproduction in Buffaloes, Caserta, Italy.