Assessment of buffalo semen preservability upon using tris-egg yolk extender enriched with different concentrations of taurine


  • Islam El-Sayed El-Seadawy Department of Animal Reproduction and Artificial Insemination, National Research Centre, Cairo, Egypt
  • Mohamed Ismail Shahba Department of Animal Reproduction and Artificial Insemination, National Research Centre, Cairo, Egypt
  • Reda Ibrahim El-Sheshtawy Department of Animal Reproduction and Artificial Insemination, National Research Centre, Cairo, Egypt
  • Walid Said El-Nattat Department of Animal Reproduction and Artificial Insemination, National Research Centre, Cairo, Egypt
  • Gamal Attia El-Sisy Department of Animal Reproduction and Artificial Insemination, National Research Centre, Cairo, Egypt


Bubalus bubalis, buffaloes, semen, freezing, taurine


Cryopreservation is connected with increased undesirable premature sperm capacitation, reduced lifespan and fertility results. Taurine acts as an antioxidant improving the semen quality after cryopreservation. The objective of the current study is to assess the effect of addition of different levels of taurine amino acid to tris-extender on bull sperm cryo-preservability. Methods: The collected semen samples were pooled and diluted with tris-citric acid-fructose egg yolk extender (control, 0% taurine) and different concentrations of taurine (10 mM, 20 Mm, 30 Mm, 40 Mm, 50 Mm, 60 Mm, 70 Mm, 80 Mm, 90 Mm and 100 mM) to final concentration of 60 x106 motile sperm/ ml. Diluted semen was processed for freezing and evaluated for sperm parameters. Results: Data showed that taurine enriched extender improved sperm parameters during cooling. Post thawing sperm motility %, membrane integrity %, Alive sperm % improved and the percentages of abnormal sperm was lowered upon using taurine enriched extender. Conclusion: It could be concluded that, most the concentrations of taurine in Tris–citrate egg yolk extender ameliorated the post cooling and post freezing semen quality in buffalo bulls.


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How to Cite

El-Seadawy, I. E.-S., Shahba, M. I., El-Sheshtawy, R. I., El-Nattat, W. S., & El-Sisy, G. A. (2021). Assessment of buffalo semen preservability upon using tris-egg yolk extender enriched with different concentrations of taurine. Buffalo Bulletin, 40(3), 485–493. Retrieved from



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