Assessment of buffalo semen preservability upon using tris-egg yolk extender enriched with different concentrations of taurine

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Islam El-Sayed EL-Seadawy Mohamed Ismail Shahba Reda Ibrahim El-Sheshtawy Walid Said El-Nattat Gamal Attia El-Sisy


Cryopreservation is connected with increased undesirable premature sperm capacitation, reduced lifespan and fertility results. Taurine acts as an antioxidant improving the semen quality after cryopreservation. The objective of the current study is to assess the effect of addition of different levels of taurine amino acid to tris-extender on bull sperm cryo-preservability. Methods: The collected semen samples were pooled and diluted with tris-citric acid-fructose egg yolk extender (control, 0% taurine) and different concentrations of taurine (10 mM, 20 Mm, 30 Mm, 40 Mm, 50 Mm, 60 Mm, 70 Mm, 80 Mm, 90 Mm and 100 mM) to final concentration of 60 x106 motile sperm/ ml. Diluted semen was processed for freezing and evaluated for sperm parameters. Results: Data showed that taurine enriched extender improved sperm parameters during cooling. Post thawing sperm motility %, membrane integrity %, Alive sperm % improved and the percentages of abnormal sperm was lowered upon using taurine enriched extender. Conclusion: It could be concluded that, most the concentrations of taurine in Tris–citrate egg yolk extender ameliorated the post cooling and post freezing semen quality in buffalo bulls.

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EL-SEADAWY, Islam El-Sayed et al. Assessment of buffalo semen preservability upon using tris-egg yolk extender enriched with different concentrations of taurine. Buffalo Bulletin, [S.l.], v. 40, n. 3, p. 485-493, sep. 2021. ISSN 2539-5696. Available at: <>. Date accessed: 21 oct. 2021.
Original Article


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