Assessment of buffalo semen preservability upon using tris-egg yolk extender enriched with different concentrations of taurine
Keywords:
Bubalus bubalis, buffaloes, semen, freezing, taurineAbstract
Cryopreservation is connected with increased undesirable premature sperm capacitation, reduced lifespan and fertility results. Taurine acts as an antioxidant improving the semen quality after cryopreservation. The objective of the current study is to assess the effect of addition of different levels of taurine amino acid to tris-extender on bull sperm cryo-preservability. Methods: The collected semen samples were pooled and diluted with tris-citric acid-fructose egg yolk extender (control, 0% taurine) and different concentrations of taurine (10 mM, 20 Mm, 30 Mm, 40 Mm, 50 Mm, 60 Mm, 70 Mm, 80 Mm, 90 Mm and 100 mM) to final concentration of 60 x106 motile sperm/ ml. Diluted semen was processed for freezing and evaluated for sperm parameters. Results: Data showed that taurine enriched extender improved sperm parameters during cooling. Post thawing sperm motility %, membrane integrity %, Alive sperm % improved and the percentages of abnormal sperm was lowered upon using taurine enriched extender. Conclusion: It could be concluded that, most the concentrations of taurine in Tris–citrate egg yolk extender ameliorated the post cooling and post freezing semen quality in buffalo bulls.
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References
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Bottrel, M., D. Acha, I. Ortiz, M. Hidalgo, J. Gósalvez, J. Camisão and J. Dorado. 2018. Cryoprotective effect of glutamine, taurine, and proline on post-thaw semen quality and DNA integrity of donkey spermatozoa. Anim. Reprod. Sci., 189: 128-135. DOI: 10.1016/j.anireprosci.2017.12.021
Bucak, M.N., A. Atessahin, O. Varıslı, A. Yuce, N. Tekin and A. Akcay. 2007. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: Microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology, 67(5): 1060-1067. DOI: 10.1016/j.theriogenology.2006.12.004
Bucak, M.N., A. Atessahin and A. Yuce. 2008. Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze-thawing process. Small Ruminant Res., 75(2-3): 128-134. DOI: 10.1016/j.smallrumres.2007.09.002
Cassani, P., M.T. Beconi and C. O’Flaherty. 2005. Relationship between total superoxide dismutase activity with lipid peroxidation, dynamics and morphological parameters in canine semen. Anim. Reprod. Sci., 86(1-2): 163-173. DOI: 10.1016/j.anireprosci.2004.06.006
Chen, Y., R.H. Foote and C.C. Brockett. 1993. Effect of sucrose, trehalose, hypotaurine, taurine, and blood serum on survival of frozen bull sperm. Cryobiology, 30(4): 423-431. DOI: 10.1006/cryo.1993.1042
de Paz, P., M.C. Esteso, M. Alvarez, M. Mata, C.A. Chamorro and L. Anel. 2010. Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology, 74(4): 663-671. DOI: 10.1016/j.theriogenology.2010.03.022
El-Sheshtawy, R.I., W.S. El-Nattat and G.A. El-Sisy. 2017. Preservability of bull spermatozoa in Tris-egg yolk extender enriched with different concentrations of butylated hydroxytoluene. Asian Pacific Journal of Reproduction, 6(2): 77-80. DOI:10.12980/apjr.6.20170205
Foote, R.H., C.C. Brockett and M.T. Kaproth. 2002. Motility and fertility of bull sperm in whole milk extender containing antioxidants. Anim. Reprod. Sci., 71(1-2): 13-23. DOI: 10.1016/s0378-4320(02)00018-0
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Jeyendran, R.S., H.H. Vander Ven, M. Perez Pelaez, B.G. Crabo and L.J.D. Zaneveld. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70(1): 219-228. DOI: 10.1530/jrf.0.0700219
Liu, Z., R.H. Foote and C.C. Brockett. 1998. Survival of bull sperm frozen at different rates in media varying in osmolarity. Cryobiology, 37(3): 219-230. DOI: 10.1006/cryo.1998.2117
Medeiros, C.M., F. Forell, A.T. Oliveira and J.L. Rodrigues. 2002. Current status of sperm cryopreservation: why isn’t it better? Theriogenology, 57(1): 327-344. DOI: 10.1016/s0093-691x(01)00674-4
Purdy, P.H. 2006. A review on goat sperm cryopreservation. Small Ruminant Res., 63(3): 215-225. DOI: 10.1016/j.smallrumres.2005.02.015
Reddy, N.S.S., G.J. Mohanarao and S.K. Atreja. 2010. Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim. Reprod. Sci., 119(3-4): 183-190. DOI: 10.1016/j.anireprosci.2010.01.012
Roof, D.J., S. Bowley, L.L. Price and D.J. Matsas. 2012. Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing. Theriogenology, 77(2): 412-420. DOI: 10.1016/j.theriogenology.2011.08.015
Saleh, A. and A. Agarwal. 2002. Oxidative stress and male infertility: From research bench to clinical practice. J. Androl., 23(6): 737-752. DOI: 10.1002/j.1939-4640.2002.tb02324.x
Sarıozkan, S., M.N. Bucak, P.B. Tuncer, P.A. Ulutas and A. Bilgen. 2009. The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology, 58(2): 134-138. DOI: 10.1016/j.cryobiol.2008.11.006
Seify, M., M. Zarabadipoura, L.K. Ghaleno, A.Z. Alizadeh and M.R. Valojerd. 2019. The anti-oxidant roles of Taurine and Hypotaurine on acrosome integrity, HBA and HSPA2 of the human sperm during vitrification and post warming in two different temperature. Cryobiology, 90: 89-95. DOI: 10.1016/j.cryobiol.2019.07.004
Sidhu, K.S. and S.S. Guraya. 1985. Buffalo Bull Semen Morphology, Biochemistry, Physiology and Methodology. Publishers and Distributors, Ludhiana, USA. p. 152-154.
SPSS. 2005. Version 14.0 for Windows Evaluation Version Release 14.0.0. Graham EF. Statistical Package for the Social Science.
Storey, B.T., E.E. Noiles and K.A. Thompson. 1998. Comparison of glycerol, other polyols, trehalose and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology, 37(1): 46-58. DOI: 10.1006/cryo.1998.2097
Upreti, G.C., K. Jensen, R. Munday, D.M. Duganzich, R. Vishwanath and J.F. Smith. 1998. Studies on aromatic amino acid oxidase activity in ram spermatozoa: role of pyruvate as an antioxidant. Anim. Reprod. Sci., 51(4): 275-287. DOI: 10.1016/s0378-4320(98)00082-7
Uysal, O., M.N. Bucak, I. Yavas and O. Varısli. 2007. Effect of various antioxidants on the quality of frozen–thawed bull semen. J. Anim. Vet. Adv., 6(12): 1362-1366. Available on: http://docsdrive.com/pdfs/medwelljournals/javaa/2007/1362-1366.pdf
Watson, P.F. 1975. Use of a giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet. Rec., 97(1): 12-15. DOI: 10.1136/vr.97.1.12
Alvarez, J.G. and B.T. Storey. 1983. Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biol. Reprod., 29(3): 548-555. DOI: 10.1095/biolreprod29.3.548
Baumber, J., B.A. Ball, C.G. Gravance, V. Medina and M.C.G. Davies-Morel. 2000. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. J. Androl., 21(6): 895-902. Available on: https://onlinelibrary.wiley.com/doi/pdf/10.1002/j.1939-4640.2000.tb03420.x
Bilodeau, J.F., S. Blanchette, I.C. Gagnon and M.A. Sirard. 2001. Thiols prevent H2O2- mediated loss of sperm motility in cryopreserved bull semen. Theriogenology, 56(2): 275-286. DOI: 10.1016/S0093-691X(01)00562-3
Bottrel, M., D. Acha, I. Ortiz, M. Hidalgo, J. Gósalvez, J. Camisão and J. Dorado. 2018. Cryoprotective effect of glutamine, taurine, and proline on post-thaw semen quality and DNA integrity of donkey spermatozoa. Anim. Reprod. Sci., 189: 128-135. DOI: 10.1016/j.anireprosci.2017.12.021
Bucak, M.N., A. Atessahin, O. Varıslı, A. Yuce, N. Tekin and A. Akcay. 2007. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: Microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology, 67(5): 1060-1067. DOI: 10.1016/j.theriogenology.2006.12.004
Bucak, M.N., A. Atessahin and A. Yuce. 2008. Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze-thawing process. Small Ruminant Res., 75(2-3): 128-134. DOI: 10.1016/j.smallrumres.2007.09.002
Cassani, P., M.T. Beconi and C. O’Flaherty. 2005. Relationship between total superoxide dismutase activity with lipid peroxidation, dynamics and morphological parameters in canine semen. Anim. Reprod. Sci., 86(1-2): 163-173. DOI: 10.1016/j.anireprosci.2004.06.006
Chen, Y., R.H. Foote and C.C. Brockett. 1993. Effect of sucrose, trehalose, hypotaurine, taurine, and blood serum on survival of frozen bull sperm. Cryobiology, 30(4): 423-431. DOI: 10.1006/cryo.1993.1042
de Paz, P., M.C. Esteso, M. Alvarez, M. Mata, C.A. Chamorro and L. Anel. 2010. Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology, 74(4): 663-671. DOI: 10.1016/j.theriogenology.2010.03.022
El-Sheshtawy, R.I., W.S. El-Nattat and G.A. El-Sisy. 2017. Preservability of bull spermatozoa in Tris-egg yolk extender enriched with different concentrations of butylated hydroxytoluene. Asian Pacific Journal of Reproduction, 6(2): 77-80. DOI:10.12980/apjr.6.20170205
Foote, R.H., C.C. Brockett and M.T. Kaproth. 2002. Motility and fertility of bull sperm in whole milk extender containing antioxidants. Anim. Reprod. Sci., 71(1-2): 13-23. DOI: 10.1016/s0378-4320(02)00018-0
Holmes, R.P., H.O. Goodman, Z.K. Shihabi and J.P. Jarow. 1992. The taurine and hypotaurine content of human semen. J. Androl., 13(3): 289-292. DOI: 10.1002/j.1939-4640.1992.tb00317.x
Jeyendran, R.S., H.H. Vander Ven, M. Perez Pelaez, B.G. Crabo and L.J.D. Zaneveld. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70(1): 219-228. DOI: 10.1530/jrf.0.0700219
Liu, Z., R.H. Foote and C.C. Brockett. 1998. Survival of bull sperm frozen at different rates in media varying in osmolarity. Cryobiology, 37(3): 219-230. DOI: 10.1006/cryo.1998.2117
Medeiros, C.M., F. Forell, A.T. Oliveira and J.L. Rodrigues. 2002. Current status of sperm cryopreservation: why isn’t it better? Theriogenology, 57(1): 327-344. DOI: 10.1016/s0093-691x(01)00674-4
Purdy, P.H. 2006. A review on goat sperm cryopreservation. Small Ruminant Res., 63(3): 215-225. DOI: 10.1016/j.smallrumres.2005.02.015
Reddy, N.S.S., G.J. Mohanarao and S.K. Atreja. 2010. Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim. Reprod. Sci., 119(3-4): 183-190. DOI: 10.1016/j.anireprosci.2010.01.012
Roof, D.J., S. Bowley, L.L. Price and D.J. Matsas. 2012. Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing. Theriogenology, 77(2): 412-420. DOI: 10.1016/j.theriogenology.2011.08.015
Saleh, A. and A. Agarwal. 2002. Oxidative stress and male infertility: From research bench to clinical practice. J. Androl., 23(6): 737-752. DOI: 10.1002/j.1939-4640.2002.tb02324.x
Sarıozkan, S., M.N. Bucak, P.B. Tuncer, P.A. Ulutas and A. Bilgen. 2009. The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology, 58(2): 134-138. DOI: 10.1016/j.cryobiol.2008.11.006
Seify, M., M. Zarabadipoura, L.K. Ghaleno, A.Z. Alizadeh and M.R. Valojerd. 2019. The anti-oxidant roles of Taurine and Hypotaurine on acrosome integrity, HBA and HSPA2 of the human sperm during vitrification and post warming in two different temperature. Cryobiology, 90: 89-95. DOI: 10.1016/j.cryobiol.2019.07.004
Sidhu, K.S. and S.S. Guraya. 1985. Buffalo Bull Semen Morphology, Biochemistry, Physiology and Methodology. Publishers and Distributors, Ludhiana, USA. p. 152-154.
SPSS. 2005. Version 14.0 for Windows Evaluation Version Release 14.0.0. Graham EF. Statistical Package for the Social Science.
Storey, B.T., E.E. Noiles and K.A. Thompson. 1998. Comparison of glycerol, other polyols, trehalose and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology, 37(1): 46-58. DOI: 10.1006/cryo.1998.2097
Upreti, G.C., K. Jensen, R. Munday, D.M. Duganzich, R. Vishwanath and J.F. Smith. 1998. Studies on aromatic amino acid oxidase activity in ram spermatozoa: role of pyruvate as an antioxidant. Anim. Reprod. Sci., 51(4): 275-287. DOI: 10.1016/s0378-4320(98)00082-7
Uysal, O., M.N. Bucak, I. Yavas and O. Varısli. 2007. Effect of various antioxidants on the quality of frozen–thawed bull semen. J. Anim. Vet. Adv., 6(12): 1362-1366. Available on: http://docsdrive.com/pdfs/medwelljournals/javaa/2007/1362-1366.pdf
Watson, P.F. 1975. Use of a giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet. Rec., 97(1): 12-15. DOI: 10.1136/vr.97.1.12
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Published
2021-09-25
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El-Seadawy, I. E.-S., Shahba, M. I., El-Sheshtawy, R. I., El-Nattat, W. S., & El-Sisy, G. A. (2021). Assessment of buffalo semen preservability upon using tris-egg yolk extender enriched with different concentrations of taurine. Buffalo Bulletin, 40(3), 485–493. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/3111
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