Cryosurvival of buffalo semen extended in tris-egg yolk supplemented with a combination of different concentrations of butylated hydroxytoluene and taurine

Main Article Content

Reda Ibrahim El-Sheshtawy

Abstract

Cryopreservation is accompanied with much detrimental premature sperm capacitation, decreased livability and fertility consequences. Taurine is considered as an antioxidant ameliorating the semen value post freeze-thawing. The purpose of this investigation was to elucidate the impact of Tris-diluent enriched with a mixture of taurine and different concentrations of butylated hydroxytoluene on buffalo bull semen preservability. Semen samples were extended in a Tris extender. Variable concentrations of butylated hydroxytoluene (BHT) (0.5, 1.0 and 2.0 mM) were set in ethyl alcohol in prewarmed (37oC) test tubes. Then taurine (60 mM) was added into each tube. Extended semen samples were added into the test tubes and put at 37oC for 5 minutes to permit the permeation of BHT into the spermatozoa. The control test tubes were Tris extender with (zero BHT and zero taurine). The test tubes were exposed to the freezing protocol. Semen was assessed and conception rate was carried out. The sperm membrane integrity (HOST) was eminently higher in TTB1 and TTB2 if compared to the control post cooling (Table 1). The post thawing results (Table 2) revealed significant improvement in sperm motility in TTB2 if compared to the control and other concentrations. Alive sperm % was kept in all concentrations as the control. Sperm abnormalities were kept in TTB2 and TTB3 as the control. Sperm membrane integrity (HOST) was significantly improved in all significantly as compared to the control. The acrosomal integrity was kept in TTB1 and TTB2 concentrations the same as the control The conception percent (Table 3) was higher in TTB2. It could be concluded that, the post cooling results exhibited the best semen quality in TTB1and TTB2. The post -thawing results revealed that, most improved sperm parameters and conception rate were in TTB2.

Downloads

Download data is not yet available.

Article Details

How to Cite
EL-SHESHTAWY, Reda Ibrahim. Cryosurvival of buffalo semen extended in tris-egg yolk supplemented with a combination of different concentrations of butylated hydroxytoluene and taurine. Buffalo Bulletin, [S.l.], v. 41, n. 3, p. 403-415, sep. 2022. ISSN 2539-5696. Available at: <https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/4742>. Date accessed: 02 dec. 2022.
Section
Original Article

References

Agarwal, A., K. Makker and R. Sharma. 2008. Clinical relevance of oxidative stress in male factor infertility: An update. Am. J. Reprod. Immunol., 59(1): 2-11. DOI: 10.1111/j.1600-0897.2007.00559.x

Aitken, R.J. and C. Krausz. 2001. Oxidative stress, DNA damage and the Y chromosome. Reproduction, 122(4): 497-506. DOI: 10.1530/reprod/122.4.497

Aitken, R.J., E. Gordon, D. Harkiss, J.P. Twigg, P. Milne, Z. Jennigs and D.S. Irvine. 1998. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol. Reprod., 59(5): 1037-1046. DOI: 10.1095/biolreprod59.5.1037

Alvarez, J.G. and B.T. Storey. 1983. Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biol. Reprod., 29(3): 548-555. DOI: 10.1095/biolreprod29.3.548

Anderson, S., W. Harkness, Y. Akin, M. Kaproth and G. Killian. 1994. Categorical data analysis of the effect on bull fertility of butylated hydroxytoluene addition to semen extenders prior to freezing. J. Dairy Sci., 77(8): 2302-2307. DOI: 10.3168/jds.S0022-0302(94)77173

Andreea, A. and Z. Stela. 2010. Role of antioxidant additives in the protection of the cryopreserved semen against free radicals. Rom. Biotech. Lett., 15(3): 3-8.

Atessahin, A., M.N. Bucak, P.B. Tuncer and M. Kızıl. 2008. Effects of anti-oxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze-thawing process. Small Ruminant Res., 77(1): 38-44. DOI: 10.1016/j.smallrumres.2008.03.002

Aumuller, G. and J. Seitz. 1990. Protein secretion and secretory processes in male accessory sex glands. Int. Rev. Cytol., 121: 127-231. DOI: 10.1016/s0074-7696(08)60660-9

Awda, B.J., M. Mackenzie-Bell and M.M. Buhr. 2009. Reactive oxygen species and boar sperm function. Biol. Reprod., 81(3): 553-555. DOI: 10.1095/biolreprod.109.076471

Ball, B.A., V. Medina, C.G. Gravance and J. Bumber. 2001. Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5oC. Theriogenology, 56(4): 577-589. DOI: 10.1016/S0093-691X(01)00590-8

Barroso, G., M. Morshedi and S. Oehninger. 2000. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum. Reprod., 15(6): 1338-1344. DOI: 10.1093/humrep/15.6.1338

Baumber, J., B.A. Ball, C.G. Gravance, V. Medina and M.C.G. Davies-Morel. 2000. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. J. Androl., 21(6): 895-902. DOI: 10.1002/j.19394640.2000.tb03420.x

Bilodeau, J.F., S. Blanchette, C. Gagnon and M.A. Sirard. 2001. Thiols prevent H2O2- mediated loss of sperm motility in cryopreserved bull semen. Theriogenology, 56(2): 275-286. DOI: 10.1016/S0093-691X(01)00562-3

Bilodeau, J.F., S. Blanchette, I.C. Gagnon and M.A. Sirard. 2001. Thiols prevent H2O2- mediated loss of sperm motility in cryopreserved bull semen. Theriogenology, 56(2): 275-286. DOI: 10.1016/S0093-691X(01)00562-3

Bucak, M.N. and N. Tekin. 2007. Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Res., 73(1-3): 103-108. DOI: 10.1016/j.smallrumres.2006.12.001

Bucak, M.N., A. Atessahin and A. Yuce. 2008. Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze-thawing process. Small Ruminant Res., 75(2-3): 128-134. DOI: 10.1016/j.smallrumres.2007.09.002

Bucak, M.N., S. Ates, A. Ahin, O. Varısli, A. Yuce, N. Tekin and A. Akcay. 2007. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology, 67(5): 1060-1067. DOI: 10.1016/j.theriogenology.2006.12.004

Cassani, P., M.T. Beconi and C. O’Flaherty. 2005. Relationship between total superoxide dismutase activity with lipid peroxidation, dynamics and morphological parameters in canine semen. Anim. Reprod. Sci., 86(1-2): 163-173. DOI: 10.1016/j.2004.06.006

Chatterjee, S., E. de Lamirande and C. Gagnon. 2001. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione. Mol. Reprod. Dev., 60(4): 498-506. DOI: 10.1002/mrd.1115

Chen, Y., R.H. Foote and C.C. Brockett. 1993. Effect of sucrose, trehalose, hypotaurine, taurine, and blood serum on survival of frozen bull sperm. Cryobiology, 30(4): 423-431. DOI: 10.1006/cryo.1993.1042

de Lamirande, E. and C. Gagnon. 1992a. Reactive oxygen species and human spermatozoa. I. Effects on the motility of intact spermatozoa and on sperm axonemes. J. Androl., 13(5): 368-378.

de Paz, P., M.C. Esteso, M. Alvarez, M. Mata, C.A. Chamorro and L. Anel. 2010. Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology, 74(4): 663-671. DOI: 10.1016/j.theriogenology.2010.03.022

El-Sisy, G.A., W.S. El-Nattat and R.I. El-Sheshtawy. 2007. Buffalo semen quality, antioxidants andperoxidation during chilling and cryopreservation. Online journal of Veterinary Research, 11: 55-61.

Ferrusola, C.O., L.G. Fernandez, B.M. Garcia, C.S. Sandoval, A.M. Rodriguez, H.R. Martinez, J.A. Tapia and F.J. Pena. 2009. Effect of cryopreservation on nitric oxide production by stallion spermatozoa. Biol. Reprod., 81(6): 1106-1111. DOI: 10.1095/biolreprod.109.078220

Foote, R.H., C.C. Brockett and M.T. Kaproth. 2002. Motility and fertility of bull sperm in whole milk extender containing antioxidants. Anim. Reprod. Sci., 71(1-2): 13-23. DOI: 10.1016/s0378-4320(02)00018-0

Fujisawa, S., Y. Kadomab and I. Yokoe. 2004. Radical-scavenging activity of butylated hydroxytoluene (BHT) and its metabolites. Chem. Phys. Lipids., 130(2): 189-195. DOI: 10.1016/j.chemphyslip.2004.03.005

Funahashi, H. and T. Sano. 2005. Selected antioxidants improve the function of extended boar semen stored at 10oC. Theriogenology, 63(6): 1605-1616. DOI: 10.1016/j.theriogenology.2004.06.016

Gallon, F., C. Marchetti, N. Jouy and P. Marchetti. 2006. The functionality of mitochondria differentiates human spermatozoa with high and low fertilizing capability. Fertil. Steril., 86(5): 1526-1530. DOI: 10.1016/j.fertnstert.2006.03.055

Graham, E.F., M.K.L. Schmehl and M. Maki-Laurila. 1970. Some physical and chemical methods of evaluating semen. p. 44-48. In Proceedings 3rd Technical Conference on Artificial Insemination and Reproduction, The National Architectural Accrediting Board (NAAB), Chicago, Illinois, USA.

Graham, J.K. and R.H. Hammerstedt. 1992. Differential effects of butylated hydroxytoluene analogs on bull sperm subjected to cold-induced membrane stress. Cryobiology, 29(1): 106-117. DOI: 10.1016/0011-2240(92)90010-y

Gutierrez-Perez, O., M.L. Juarez-Mosqueda, S.U. Carvajal and M.E.T. Ortega. 2009. Boar spermatozoa cryopreservation in low glycerol/trehalose enriched freezing media improves cellular integrity. Cryobiology, 58(3): 287-292. DOI: 10.1016/j.cryobiol.2009.02.003

Halliwell, B. 2006. Oxidative stress and neurodegeneration: Where are we now?. J. Neurochem., 97(6): 1634-1658. DOI: 10.1111/j.1471-4159.2006.03907.x

Halliwell, B. and J. Gutteridge. 2007. Free Radicals in Biology and Medicine, 4th ed. Press, New York, USA.

Hammerstedt, R.H., R.P. Amann, T. Rucinsky, P.D. Morse, J. Lepock and W. Snipes. 1976. Use of spin labels and electron spin resonance spectroscopy to characterize membranes of bovine sperm: Effect of butylated hydroxytoluene and cold shock. Biol. Reprod., 14(4): 381-397. DOI: 10.1095/biolreprod14.4.381

Hu, J.H., Q.W. Li, G. Li, Z.L. Jiang, S.H. Bu, H. Yang and L.Q. Wang. 2009. The cryoprotective effect of trehalose supplementation on boar spermatozoa quality. Anim. Reprod. Sci., 112(1-2): 107-118. DOI: 10.1016/j.anireprosci.2008.04.009

Ijaz, A., A. Hussain, M. Aleem, M.S. Yousaf and H. Rehman. 2009. Butylated hydroxytoluene inclusion in semen extender improves the post thawed semen quality of Nili-Ravi buffalo (Bubalus bubalis). Theriogenology, 71(8): 1326-1329. DOI: 10.1016/j.theriogenology.2008.12.023

Jeyendran, R.S., H.H. Vander Ven, M. Perez Pelaez, B.G. Crabo and L.J.D. Zaneveld. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70(1): 219-228. DOI: 10.1530/jrf.0.0700219

Khalifa, T.A.A., A.G. Lymberopoulos and B.E. ElSaidy. 2008. Testing usability of butylated hydroxytoluene in conservation of goat semen. Reprod. Domest. Anim., 43(5): 525-530. DOI: 10.1111/j.1439-0531.2007.00947.x

Khalifa, T.A.A. and B.E. El Saidy. 2006. Pellet-freezing of damascus goat semen in a chemically defined extender. Anim. Reprod. Sci., 93(3-4): 303-315. DOI: 10.1016/j.anireprosci.2005.08.008

Khan, M.I.R. and A. Ijaz. 2007. Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays. Ital. J. Anim. Sci., 6(2): 784-787. DOI: 10.4081/ijas.2007.s2.784

Koppers, A.J., G.N. De Iuliis, J.M. Finnie, E.A. McLaughlin and R.J. Aitken. 2008. Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J. Clin. Endocr. Metab., 93(8): 3199-3207. DOI: 10.1210/jc.2007-2616

Kubkomawa, H. 2018. The use of artificial insemination (AI) technology in improving milk, beef and reproductive efficiency in tropical Africa: A review. Journal of Dairy and Veterinary Sciences, 5(2): 1-18. Available on: https://juniperpublishers.com/jdvs/pdf/JDVS.MS.ID.555660.pdf

Liu, Z., R.H. Foote and C.C. Brockett. 1998. Survival of bull sperm frozen at different rates in media varying in osmolarity. Cryobiology, 37(3): 219-230. DOI: 10.1006cryo.1998.2117

Maia, M.D.S., S.D. Bicudo, C.C. Sicherle, L. Rodello and I.C.S. Gallego. 2010. Lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen cryopreserved in extenders with antioxidants. Anim. Reprod. Sci., 122(1-2): 118-123. DOI: 10.1016/j.anireprosci.2010.08.004

Mammoto, A., N. Masumoto, M. Tahara, Y. Ikebuchi, M. Ohmichi and K. Tasaka. 1996. Reactive oxygen species block sperm-egg fusion via oxidation of sperm sulfhydryl proteins in mice. Biol. Reprod., 55(5): 1063-1068. DOI: 10.1095/biolreprod55.5.1063

Martins-Bessa, A., A. Rocha and A. Mayenco-Aguirre. 2009. Effects of taurine and hypotaurine supplementation and ionophore concentrations on post-thaw acrosome reaction of dog spermatozoa. Theriogenology, 71(2): 248-253. DOI: 10.1016/j.theriogenology.2008.07.006

Medeiros, C.M., F. Forell, A.T. Oliveira and J.L. Rodrigues. 2002. Current status of sperm cryopreservation: Why isn’t better. Theriogenology, 57(1): 327-344. DOI: 10.1016/s0093-691x(01)00674-4

Memon, A.A., H. Wahid, Y. Rosnina, Y.M. Goh, M. Ebrahimi and F.M. Nadia. 2012. Effect of antioxidants on post thaw microscopic, oxidative stress parameter and fertility of Boer goat spermatozoa in Tris egg yolk glycerol extender. Anim. Reprod. Sci., 136(1-2): 55-60. DOI: 10.1016/j.anireprosci.2012.10.020

Michael, A., C. Alexopoulos, E. Pontiki, D. Hadjipavlou-Litina, P. Saratsis and C. Boscos. 2007. Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology, 68(2): 204-212. DOI: 10.1016/j.theriogenology.2007.04.053

Mittal, P.K., A.K. Madan, V. Sharma, G.S. Gottam and B. Gupta. 2019. Cryopreservation of buffalo bull semen-restriction and expectation: A review. International Journal of Current Microbiology and Applied Sciences, 8(1): 1351-1368. DOI: 10.20546/ijcmas.2019.801.144

Mughal, D.H., A. Ijaz, M.S. H. Yousaf, M. Rehman, H. Aleem and Zaneb. 2013. Assessment of optimal osmotic pressure of citrate egg yolk extender for cryopreservation of buffalo bull (Bubalus bubalis) semen. J Anim Plant Sci., 23(4): 964-968. Available on: http://thejaps.org.pk/docs/v-23-4/03.pdf

Neagu, V.R., B.M. Garcia, C.S. Sandoval, A.M. Rodriguez, C.O. Ferrusola, L.G. Fernandez, J.A. Tapia and F.J. Pena. 2010. Freezing dog semen in presence of the antioxidant butylatedhy droxytoluene improves postthaw sperm membrane integrity. Theriogenology, 73(5): 645-650. DOI: 10.1016/j.theriogenology.2009.10.021

Neild, D.M., B.M. Gadella, M.G. Chaves, M.H. Miragaya, B. Colenbrander and A. Aguero. 2003. Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation. Theriogenology, 59(8): 1693-1705. DOI: 10.1016/S0093-691X(02)01231-1

O’Flaherty, C. 2014. Iatrogenic genetic damage of spermatozoa. In Baldi, E. and M. Muratori (eds.) Genetic Damage in Human Spermatozoa, Springer, New York, USA.

O’Flaherty, C., N. Beorlegui and M.T. Beconi. 2003. Participation of superoxide anion in the capacitation of cryopreserved bovine sperm. Int. J. Androl., 26(2): 109-114. DOI: 10.1046/j.1365-2605.2003.00404.x

Patel, A., A. Saxena, D.K. Swain, D. Yadav, S.S. Yadav, A. Kumar and A. Kumar. 2015. Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls. Vet. World, 8(6): 808-812. DOI: 10.14202/vetworld.2015.808-812

Purdy, P.H. 2006. A review on goat sperm cryopreservation. Small Ruminant Res., 6(3): 215-225. DOI: 10.1016/j. smallrumres.2005.02.015

Pursel, V.G. 1979. Effect of cold shock on boar sperm treated with butylated hydroxytoluene. Biol. Reprod., 21(2): 319-324. DOI: 10.1095/biolreprod21.2.319

Reddy, N.S.S., G.J. Mohanarao and S.K. Atreja. 2010. Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim. Reprod. Sci., 119(3-4): 183-190. DOI: 10.1016/j.anireprosci.2010.01.012

Roca, J., M.A. Gil, M. Hernandez, I. Parrilla, J.M. Vazquez and E.A. Martinez. 2004. Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene. J. Androl., 25(3): 397-405. DOI: 10.1002/j.1939-4640.2004.tb02806.x

Roof, D.J., S. Bowley, L.L. Price and D.J. Matsas. 2012. Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing. Theriogenology, 77(2): 412-420. DOI: 10.1016/j.theriogenology.2011.08.015

Saleh, A. and A. Agarwal. 2002. Oxidative stress and male infertility: From research bench to clinical practice. J. Androl., 23(6): 737-752. DOI: 10.1002/j.1939-4640.2002.tb02324.x

Sarıozkan, S., M.N. Bucak, P.B. Tuncer, P.A. Ulutas and A. Bilgen. 2009. The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology, 58(2): 134-138. DOI: 10.1016/j.cryobiol.2008.11.006

Shoae, A. and M.J. Zamiri. 2008. Effect of butylated hydroxytoluene on bull spermatozoa frozen in egg yolk-citrate extender. Anim. Reprod. Sci., 104(2-4): 414-418. DOI: 10.1016/j.anireprosci.2007.07.009.

Sidhu, K.S. and S.S. Guraya. 1985. Buffalo Bull Semen: Morphology, Biochemistry, Physiology and Methodology. USG Publishers and Distributors, Ludhiana, USA. 184p.

Smith, R., H. Kaune, D. Parodi, M. Madariaga, R. Rios, I.A. MoralesCastro. 2006. Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress. Hum. Reprod., 21(4): 986-993. DOI: 10.1093/humrep/dei429

SPSS. 2005. Version 14.0 for Windows Evaluation Version Release 14.0.0. In Graham, E.F. Statistical Package for The Social Science, Chicago, USA.

Storey, B.T., E.E. Noiles and K.A. Thompson. 1998. Comparison of glycerol, other polyols, trehalose and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology, 37(1): 46-58. DOI: 10.1006/cryo.1998.2097

Stradaioli, G., T. Noro, L. Sylla and M. Monaci. 2007. Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: Comparison between two extenders. Anim. Reprod. Sci., 67(7): 1249-1255. DOI: 10.1016/j.theriogenology.2007.01.009

Thomas, A.D., S.A. Meyers and B.A. Ball. 2006. Capacitation-Like changes in equine spermatozoa following cryopreservation. Theriogenology, 65(8): 1531-1550. DOI: 10.1016/j.theriogenology.2005.08.022

Thomson, L.K., S.D. Fleming, R.J. Aitken, G.N. De luliis, J.A. Zieschang and A.M. Clark. 2009. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum. Reprod., 24(9): 2061-2070. DOI: 10.1093/humrep/dep214.

Tremellen, K. 2008. Oxidative stress and male infertility - A clinical perspective. Hum. Reprod., 14(3): 243-258. DOI: 10.1093/humupd/dmn004

Upreti, G.C., K. Jensen, R. Munday, D.M. Duganzich, R. Vishwanath and J.F. Smith. 1998. Studies on aromatic amino acid oxidase activity in ram spermatozoa: Role of pyruvate as an antioxidant. Anim. Reprod. Sci., 51(4): 275-287. DOI: 10.1016/s0378-4320(98)00082-7.

Uysal, O., M.N. Bucak, I. Yavas and O. Varıslı. 2007. Effect of various antioxidants on the quality of frozen-thawed bull semen. J. Anim. Vet. Adv., 6(12): 1362-1366. Available on: http://docsdrive.com/pdfs/medwelljournals/javaa/2007/1362-1366.pdf.

Vishwanath, R. and P. Shannon. 2000. Storage of bovine semen in liquid and frozen state. Anim. Reprod. Sci., 62(1-3): 23-53. DOI: 10.1016/S0378-4320(00)00153-6.

Watson, P.F. 2000. The cause of reduced fertility with cryopreserved semen. Anim. Reprod. Sci., 61: 481-492. DOI: 10.1016/S0378-4320(00)00099-3.

Watson, P.F. 1975. Use of giemsa stain to detect changes in the acrosome of frozen ram spermatozoa. Vet. Record., 97(1): 12-15. DOI: 10.1136/vr.97.1.12.

Williams, G.M., M.J. Iatropoulos and J. Whysner. 1999. Safety assessment of butylated hydroxyanisole and butylated hydroxytoluene as antioxidant food additives. Food Chem. Toxicol., 37(9-10): 1027-1038. DOI: 10.1016/S0278-6915(99)00085-X.