Cryosurvival of buffalo semen extended in tris-egg yolk supplemented with a combination of different concentrations of butylated hydroxytoluene and taurine

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Reda Ibrahim El-Sheshtawy


Cryopreservation is accompanied with much detrimental premature sperm capacitation, decreased livability and fertility consequences. Taurine is considered as an antioxidant ameliorating the semen value post freeze-thawing. The purpose of this investigation was to elucidate the impact of Tris-diluent enriched with a mixture of taurine and different concentrations of butylated hydroxytoluene on buffalo bull semen preservability. Semen samples were extended in a Tris extender. Variable concentrations of butylated hydroxytoluene (BHT) (0.5, 1.0 and 2.0 mM) were set in ethyl alcohol in prewarmed (37oC) test tubes. Then taurine (60 mM) was added into each tube. Extended semen samples were added into the test tubes and put at 37oC for 5 minutes to permit the permeation of BHT into the spermatozoa. The control test tubes were Tris extender with (zero BHT and zero taurine). The test tubes were exposed to the freezing protocol. Semen was assessed and conception rate was carried out. The sperm membrane integrity (HOST) was eminently higher in TTB1 and TTB2 if compared to the control post cooling (Table 1). The post thawing results (Table 2) revealed significant improvement in sperm motility in TTB2 if compared to the control and other concentrations. Alive sperm % was kept in all concentrations as the control. Sperm abnormalities were kept in TTB2 and TTB3 as the control. Sperm membrane integrity (HOST) was significantly improved in all significantly as compared to the control. The acrosomal integrity was kept in TTB1 and TTB2 concentrations the same as the control The conception percent (Table 3) was higher in TTB2. It could be concluded that, the post cooling results exhibited the best semen quality in TTB1and TTB2. The post -thawing results revealed that, most improved sperm parameters and conception rate were in TTB2.


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EL-SHESHTAWY, Reda Ibrahim. Cryosurvival of buffalo semen extended in tris-egg yolk supplemented with a combination of different concentrations of butylated hydroxytoluene and taurine. Buffalo Bulletin, [S.l.], v. 41, n. 3, p. 403-415, sep. 2022. ISSN 2539-5696. Available at: <>. Date accessed: 02 dec. 2022.
Original Article


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