Comparative study of Tris Turmeric, Tris Turmeric Dimethyl Sulfoxide and Tris Turmeric Ethylene glycol extenders on the cryosurvivability, sperm resistance, in- vivo fertility and antioxidant status in buffalo bull semen
DOI:
https://doi.org/10.56825/bufbu.2024.4335105Keywords:
Bubalus bubalis, buffaloes, Tris turmeric, Tris turmeric dimethyl sulfoxide, Tris turmeric ethylene glycol, Giza, EgyptAbstract
The freeze-thaw process leads to structural and functional damage due to excessive accumulation of reactive oxygen species (ROS). The addition of exogenous antioxidants to sperm diluents is of great importance to overcome oxidative damage during freezing. The purpose of this study was to explore the effects of three diluents Tris Turmeric, Tris Turmeric Dimethyl Sulfoxide and Tris Turmeric Ethylene glycol on the cold survival ability of buffalo sperm. Semen was collected from five local adult male buffalo breeds. A base diluent of Tris-citric acid-fructose (TCF) was prepared, adding 20% whole egg yolk (TCFY). The Tris extender without turmeric, without DMSO, and without EG was kept as a control. Other extenders are Tris containing turmeric TT (100 ml/5 ml Tris), Tris containing turmeric dimethyl sulfoxide TTD (100 ml/5 ml Tris + 1.5% DMSO) and Tris containing turmeric and ethylene glycol TTE EG (100 ml/5 ml Tris + 1.5% EG). Semen samples were added and a pure sperm concentration of 60 × 106/ml was achieved. Frozen buffalo sperm after thawing showed significant improvements in all research parameters of the three breeding samples compared to the control. Tris Turmeric Ethylene was the type that best improved sperm survival under frozen conditions, followed by Tris Turmeric and Tris Turmeric Dimethyl Sulfoxide compared to the control. A significant decrease in sperm motility after thawing was evident as usage time increased in all expanders. There was a significant increase in total antioxidant content (TAC) and insignificant change in malondialdehyde (MDA) of the diluent used compared to the control. Conception rate (CR) was higher in Tris Turmeric Ethylene glycol (65.2%), followed by Tris Turmeric (60.3%) and Tris Turmeric Dimethyl Sulfoxide (55.9%) compared to the control (36, 7%). It can be concluded that Tris Turmeric Ethylene Glycol is considered the best agent for improving cold survival and sperm fertility, followed by Tris Turmeric and Tris Turmeric Dimethyl Sulfoxide.
Downloads
Metrics
References
Agarwal, A., S.A. Prahakaran and T.M. Said. 2005. Prevention of oxidative stress injury to sperm. J. Androl., 26(6): 654-660. DOI: 10.2164/jandrol.05016
Agarwal, A., K. Makker and R. Sharma. 2008. Clinical relevance of oxidative stress in male factor infertility: An update. Am. J. Reprod. Immunol., 59(1): 2-11. DOI: 10.1111/j.1600-0897.2007.00559.x
Akhter, S., M.S. Ansari, S.M.H. Andrabi, N. Ullah and M. Qayyum. 2008. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod. Domest. Anim., 43(3): 272-278. DOI: 10.1111/j.1439-0531.2007.00890.x
Al Naib, A., J.P. Hanarahan, P. Lonergan and S. Fair. 2011. In vitro assessment of sperm from bulls of high and low fertility. Theriogenology, 76(1): 161-167. DOI: 10.1016/j.theriogenology.2010.10.038
Andrabi, S.M. 2009. Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reprod. Domest. Anim., 44(3) :552-569. DOI: 10.1111/j.1439-0531.2008.01240.x
Bagchi, A. 2012. Extraction of curcumin. IOSR Journal of Environmental Science, Toxicology and Food Technology, 1(3): 1-16. Available on: https://www.iosrjournals.org/iosr-jestft/papers/vol1-issue3/A0130116.pdf
Bailey, J.L., J.F. Bilodeau and N. Cormier. 2000. Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon. J. Androl., 21(1):1-7.
Ball, B.A. and A. Vo. 2001. Osmotic tolerance of equine spermatozoa and the effects of soluble cryoprotectants on equine sperm motility, viability, and mitochondrial membrane potential. J. Androl., 22(6): 1061-1069. DOI: 10.1002/j.1939-4640.2001.tb03446.x
Barbas, J.P. and R.D. Mascarenhas. 2009. Cryopreservation of domestic animal sperm cells. Cell Tissue Bank., 10(1): 49-62. DOI: 10.1007/s10561-008-9081-4
Barroso, G., M. Morshedi and S. Oehninger. 2000. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum. Reprod., 15(6): 1338-1344. DOI: 10.1093/humrep/15.6.1338
Bearden, H.J. and J.W. Fuquay. 1980. Applied Animal Reproduction. Reston Publishing Company, the University of Michigan, USA, p. 158-160.
Bhowmik, D., K.P. Chiranjib, S. Kumar, M. Chandira and B. Jayakar. 2009. Turmeric: A herbal and traditional medicine. Archives of Applied Science Research, 1(2): 86-108. Available on: https://www.scholarsresearchlibrary.com/articles/turmeric-a-herbal-and-traditional-medicine.pdf
Bilodeau, J.F., S. Blanchette, I.C. Gagnon and M.A. Sirard. 2001. Thiols prevent H2O2- mediated loss of sperm motility in cryopreserved bull semen. Theriogenology, 56(2): 275-286. DOI: 10.1016/s0093-691x(01)00562-3
Bucak, M.N., N. Baspinar, P.B. Tuncer, K. Coyan, S. Sariozkan, P.P. Akalin, S. Buyukleblebici and S. Kucukgunay. 2012. Effects of curcumin and dithioerythritol on frozen-thawed bovine semen. Andrologia, 44(1): 102-109. DOI: 10.1111/j.1439-0272.2010.01146.x
Büyükleblebici, S., P.B. Tuncer, M.N. Bucak, U. Taşdemir, A. Eken, O. Büyükleblebici, E. Durmaz, S. Sarıözkan and B.Ü. Endirlik. 2014. Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders. Cryobiology, 69(1): 74-78. DOI: 10.1016/j.cryobiol.2014.05.005
Coutinho da Silva, M.A., G.E. Seidel Jr., E.L. Squires, J.K. Graham and E.M. Carnevale. 2012. Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae. Reproduction, 143(5): 577-585. DOI: 10.1530/REP-11-0099
Curry, M.R., J.D. Millar and P.F. Watson. 1994. Calculated optimal cooling rates for ram and human sperm cryopreservation fail to conform with empirical observations. Biol. Reprod., 51(5): 1014-1021. DOI: 10.1095/biolreprod51.5.1014
De Lamirande, E. and C. Gagnon. 1992. Reactive oxygen species and human spermatozoa. I. Effects on the motility of intact spermatozoa and on sperm axonemes. J. Androl., 13(5): 368-378.
El-Harairy, M.A., E.B. Laila, A.M. Eid Azeidan, Abd El-Salaam and M.A.M. El- Kishk. 2011. Quality and fertility of the frozen-thawed bull semen as affected by the different cryoprotectants and glutathione level. Am. J. Sci., 7: 791-801
Farshad, A., B. Khalili and P. Fazeli. 2009. The effect of different concentrations of glycerol and DMSO on viability of Markhoz goat spermatozoa during different freezing temperatures steps. Pakistan Journal of Biological Sciences, 12(3): 239-245. DOI: 10.3923/pjbs.2009.239.245
Ferrusola, C.O., L.G. Fernandez, B.M. Garcia, C. Salazar-Sandoval, A.M. Rodriguez, H.R. Martinez, J.A. Tapia and F.J. Pena. 2009. Effect of cryopreservation on nitric oxide production by stallion spermatozoa. Biol. Reprod., 81(6): 1106-1111. DOI: 10.1095/biolreprod.109.078220
Foote, R.H. 1970. Fertility of bull semen at high extension rates in Tris buffered extenders. J. Dairy Sci., 53(10): 1475-1477. DOI: 10.3168/jds.S0022-0302(70)86417-7
Gadea, J., D. Gumbo, S. Cánovas, F.A. García-Vázquez, L.A. Grullon and J.C. Gardon. 2007. Supplementation of the dilution medium after thawing with reduced glutathione improves function and the in vitro fertilizing ability of frozen-thawed bull spermatozoa. Int. J. Androl., 31(1): 40-49. DOI: 10.1111/j.1365-2605.2007.00756.x
Gallon, F., C. Marchetti, N. Jouy and P. Marchetti. 2006. The functionality of mitochondria differentiates human spermatozoa with high and low fertilizing capability. Fertil. Steril., 86(5): 1526-1530. DOI: 10.1016/j.fertnstert.2006.03.055
Gilmore, J.A., L.E. McGann, J. Liu, D.Y. Gao, A.T. Peter and F.W. Kleinhans. 1995. Effect of cryoprotectant solutes on water permeability of human spermatozoa. Biol. Reprod., 53(5): 985-995. DOI: 10.1095/biolreprod53.5.985
Głombik, K., A. Basta-Kaim, M. Sikora-Polaczek, M. Kubera, G. Starowicz and J. Styrna. 2014. Curcumin influences semen quality parameters and reverses the di (2-ethylhexyl)phthalate (DEHP)-induced testicular damage in mice. Pharmacol. Rep., 66(5): 782-787. DOI: 10.1016/j.pharep.2014.04.010
Guthrie, H.D. and G.R. Welch. 2006. Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J. Anim. Sci., 84(8): 2089-100. DOI: 10.2527/jas.2005-766.
Guthrie, H.D., J. Liu and J.K. Critser. 2002. Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa. Biol. Reprod., 67(6): 1811-1816. DOI: 10.1095/biolreprod67.6.1811
Halliwell, B. and J. Gutteridge. 2007. Free Radicals in Biology and Medicine, Oxford University Press, New York., USA.
Halliwell, B. 2006. Oxidative stress and neurodegeneration: Where are we now? J. Neurochem., 97(6): 1634-1658. DOI: 10.1111/j.1471-4159.2006.03907.x
Koppers, A.J., G.N. De Iuliis, J.M. Finnie, E.A. McLaughlin and R.J. Aitken. Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin. Endocr. Metab., 93(8): 3199-207. DOI: 10.1210/jc.2007-2616
Koracevic, D., G. Koracevic, V. Djordjevic, S. Andrejevic and V. Cosic. 2001. Method for the measurement of antioxidant activity in human fluids. J. Clinic. Pathol., 54(5): 356-361. DOI: 10.1136/jcp.54.5.356
Kubkomawa, H.I., S.M. Adamu, C.C. Achonwa, K.A. Adewuyi and I.C. Okoli. 2018. Beef production and marketing in Nigeria: Entrepreneurship in animal agriculture. International Journal of Veterinary Sciences and Animal Husbandry, 3(2): 26-40. Available on: https://www.veterinarypaper.com/pdf/2018/vol3issue2/PartA/3-1-15-109.pdf
Kulkarni, S.J., K.N. Maske, M.P. Budre and R.P. Mahajan. 2012. Extraction and purification of curcuminoids from Turmeric (Curcuma longa L.). International Journal of Pharmacy and Pharmaceutical Sciences, 1(2): 2277-3436.
Kuroda, K., M. Fukushima and H. harayama. 2007. Premature capacitation of frozen-thawed spermatozoa from subfertile Japanese black cattle. J. Reprod. Develop., 53(5): 1079-1086. DOI: 10.1262/jrd.19031
Li, Y., D. Kalo, Y. Zeron and Z. Roth. 2016. Progressive motility - A potential predictive parameter for semen fertilization capacity in bovines. Zygote, 24(1): 70-82. DOI: 10.1017/S0967199414000720
Mahmoud, K.G.M., A.A.E. EL Sokary, M.E.A. Abou el Roos, A.D.A. Ghafar and M. Nawito. 2013. Sperm characteristics in cryopreserved buffalo bull semen and field fertility. Iranian Journal of Applied Animal Science, 3(4): 777-783.
Massip, A. 2001. Cryopreservation of embryos of farm animals. Reprod. Domest. Anim., 36(2): 49-55. DOI: 10.1046/j.1439-0531.2001.00248.x
Mathuria, N. and R.J. Verma. 2008. Ameliorative effect of curcumin on aflatoxin-induced toxicity in serum of mice. Acta pol. Pharm., 65(3): 339-343.
Mazur, P. 1984. Freezing of living cells: mechanisms and implications. Am. J. Physiol., 247: C125-C142. DOI: 10.1152/ajpcell.1984.247.3.C125
Memon, A.A., H. Wahid, Y. Rosnina, Y.M. Goh, M. Ebrahimi, F.M. Nadia and G. Audrey. 2011. Effect of butylated hydroxytoluene on cryopreservation of Boer goat semen in Tris egg yolk extender. Anim. Reprod. Sci., 129(1-2): 44-49. DOI: 10.1016/j.anireprosci.2011.10.004
Milovanov, V.K. 1962. The biology of reproduction and artificial insemination of farm animals. Biologija Vos-Proizyedenija i Iskusstvennoe Oesemenenie Zivotnyh, Moscow, Russia. 696p.
Moore, A.I., E.L. Squires , J.E. Bruemmer and J.K. Graham. 2006 . Effect of cooling rate and cryoprotectant on the cryosurvival of equine spermatozoa. J. Equine Vet. Sci., 26(5): 215-218. DOI: 10.1016/j.jevs.2006.03.003
Nair, S.J., A.S. Brar, C.S. Ahuja, S.P. Sangha and K.C.A. Chaudhary. 2006. comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature. Anim. Reprod. Sci., 96(1-2): 21-29. DOI: 10.1016/j.anireprosci.2005.11.002
Neild, D.M., B.M. Gadella, M.G. Chaves, M.H. Miragaya, B. Colenbrander and A. Aguero. 2003. Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation. Theriogenology, 59(8): 1693-1705. DOI: 10.1016/s0093-691x(02)01231-1
Nelson, K.M., J. Dahlin, J. Bisson, J. Graham, G.F. Pauli and M.A. Walters. 2017. The essential medicinal chemistry of curcumin. J. Med. Chem., 60(5): 1620-1673. DOI: 10.1021/acs.jmedchem.6b00975
O’Flaherty, C., N. Beorlegui and M.T. Beconi. 2003. Participation of superoxide anion in the capacitation of cryopreserved bovine sperm. Int. J. Androl., 26(2): 109-14. DOI: 10.1046/j.1365-2605.2003.00404.x
Petruska, P., M.M. Capcarova and P. Sutovsky. 2014. Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species. Turk. J .Vet .Anim. Sci., 38(6): 643-652. DOI: 10.3906/vet-1404-61
Purdy, P.H. 2006. A review on goat sperm cryopreservation. Small Rum. Res., 63(3): 215-225 DOI: 10.1016/j.smallrumres.2005.02.015
Ramos, L. and A.M.M. Wetzels. 2001. Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay. Hum. Reprod., 16(8): 1703-1707.
Rasul, Z., N. Ahmad and M. Anzar. 2007. Antagonist effect of DMSO on the cryoprotection ability of glycerol during cryopreservation on buffalo sperm. Theriogenology, 68(5): 813-819. DOI: 10.1016/j.theriogenology.2007.06.014
Revell, S.G. and R.A. Mrode. 1994. An osmotic resistance test for bovine semen. Anim. Reprod. Sci., 36(1-2): 77-86. DOI: 10.1016/0378-4320(94)90055-8
Sahoo, D.K., A. Roy and G.B. Chainy. 2008. Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chem. Biol. Interact., 176(2-3): 121-128. DOI: 10.1016/j.cbi.2008.07.009
Salamon, S. and W.M. Maxwell. 2000. Storage of ram semen. Anim. Reprod. Sci. 62(1-3): 77-111. DOI: 10.1016/s0378-4320(00)00155-x
Satoh, K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta, 90(1): 37-43. DOI: 10.1016/0009-8981(78)90081-5
Seeram, N.P., L.S. Adams, M.L. Hardy and D. Heber. 2004. Total cranberry extract versus its phytochemical constituents: antiproliferative and synergistic effects. J. Agric. Food Chem., 52(9): 2512-2517. DOI: 10.1021/jf0352778
Serafini, R., V. Longobardi, M. Spadetta, D. Neri, B. Ariota, B. Gasparrini and R.D. Palo. 2014. Trypan blue/giemsa staining to assess sperm membrane integrity in salernitano stallions and its relationship to pregnancy rates. Reprod. Domest. Anim., 49(1): 41-47. DOI: 10.1111/rda.12221
Sharma, O.P. 1976. Antioxidant Activity of Curcumin and Related Compounds. Biochemical Pharmacology, 25(15): 1811-1812. DOI: 10.1016/0006-2952(76)90421-4
Shibahara, H., S. Naito, A. Hasegawa, M. Mitsuo, M. Shigeta and K. Koyama. 1997. Evaluation of sperm fertilizing ability using the Sperm Quality Analyzer, 20: 112-117.
Siemea, H., H. Oldenhofa and W.F. Wolkers. 2016. Mode of action of cryoprotectants for sperm preservation. Bubalus Bubalis, 169: 2-5. DOI: 10.1016/j.anireprosci.2016.02.004
Singh, A.K., V.K. Singh, B.M. Narwade, T.K. Mohanty and S.K. Atreja. 2012. Comparative quality assessment of buffalo (Bubalus bubalis) semen chilled (5°C) in egg yolk-and soya milk-based extenders. Reprod. Domest. Anim.; 47(4): 596-600. DOI: 10.1111/j.1439-0531.2011.01928.x
Smith, R., H. Kaune, D. Parodi, M. Madariaga, R. Rios, I. Moralesand and A. Castro. 2006. Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress. Hum. Reprod., 21(4): 986-993. DOI: 10.1093/humrep/dei429
Soares, M.P., C.A.R. Rossi and A. Mezzalira. 2002. Cecim Methylene glycol on canine semen cryopreservation. Ciencia Rural, 32: 649-655.
SPSS. 2005. Version 14.0 for Windows Evaluation Version Release 14.0.0. In Graham, E.F. (edn.) Statistical Package for The Social Science, Chicago, USA.
Vale,W.G. 1997. Sperm cryopreservation. Bubalus Bubalis, 1: 129-140.
Visconti, P.E., H. Galantino-Homer, G.D. Moore, J.L. Bailey, X. Ning, M. Fornes and G.S. Kopf. 1998. The molecular basis of sperm capacitation. J. Androl., 19(2): 242-248.
Vishwanath, R. and P. Shannon. 2000. Storage of bovine semen in liquid and frozen state. Anim. Reprod. Sci., 62(1-3): 23-53. DOI: 10.1016/s0378-4320(00)00153-6
Watson, P.F. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci., 60-61: 481-492. DOI: 10.1016/s0378-4320(00)00099-3
Yanagimachi, R. 1994. Mammalian fertilization, p. 189-317. In Knobil, E. and J.D. Neill. (eds.) The physiology of Reproduction, 2nd ed. Raven Press, NewYork, USA. DOI: 10.1093/biolre/ioac037
Downloads
Published
How to Cite
Issue
Section
.png){kind=logo}
