Effect of melatonin implantation prior to ovsynch plus cidr protocol on subsequent ovarian activity and pregnancy rate in summer anestrous Murrah buffaloes
DOI:
https://doi.org/10.56825/bufbu.2023.4223586Keywords:
Bubalus bubalis, buffaloes, melatonin, ovarian activity, pregnancy, summerAbstract
For assessing the impact of melatonin pre-treatment in summer anestrous Murrah buffaloes on subsequent reproductive activity of a fixed time artificial insemination (FTAI) protocol, 83 anestrous Murrah buffaloes were subcutaneously inserted 2x4 mm size absorbable melatonin (MT) implants (18 mg/50 kg b wt) at the base of left ear and 38 Murrah buffaloes were used as non-implanted controls. On day 35 post-MT, the Treatment and Control group Murrah buffaloes were subjected to FTAI protocol (Ovsynch plus CIDR). Ovarian ultrasonography and jugular vein blood sampling was carried out on day 7 and 10 of protocol and on day 7-post AI. Overall pregnancy rate on day 90 post-AI in MT group was higher compared to Control group (55.4 vs 31.6%, P<0.05), along with the higher (P>0.05) plasma progesterone on day 7 of FTAI protocol in Murrah buffaloes of the former group which conceived subsequently compared to their non-pregnant counterparts. Moreover, MT group had higher (P>0.05) increase in dominant follicle diameter between day 7 and 10 of protocol compared to Control group. On day 7 post-AI, the luteal profile in pregnant Murrah buffaloes of MT group was better (P<0.05) compared to their counterparts in Control group (CL: 14.80±0.70 vs 10.82±0.46 mm, P4: 1.54±0.07 vs 1.04±0.08 ng/ml). In conclusion, melatonin pre-treatment in summer anestrous Murrah buffaloes could be associated with better luteal activity and higher pregnancy rate in the Ovsynch+CIDR protocol.
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References
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Pandey, A.K., S.P.S. Ghuman, G.S. Dhaliwal, M. Honparkhe, J.B. Phogat and S. Kumar. 2018. Effects of preovulatory follicle size on estradiol concentrations, corpus luteum diameter, progesterone concentrations and subsequent pregnancy rate in buffalo cows (Bubalus bubalis). Theriogenology, 107: 57-62. DOI: 10.1016/j.theriogenology.2017.10.048
Pandey, A.K., P. Gunwant, N. Soni, Kavita, S. Kumar, A. Kumar, A. Magotra, I. Singh, J.B. Phogat, R.K. Sharma, Y. Bangar, S.P.S. Ghuman and S.S. Sahu. 2019. Genotype of MTNR1A gene regulates the conception rate following melatonin treatment in water buffalo. Theriogenology, 128: 1-7. DOI: 10.1016/j.theriogenology.2019.01.018
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Ramadan, T.A., R.K. Sharma, S.K. Phulia, A.K. Balhara, S.S. Ghuman and I. Singh. 2014. Effectiveness of melatonin and controlled internal drug release device treatment on reproductive performance of buffalo heifers during out-of-breeding season under tropical conditions. Theriogenology, 82(9): 1296-1302. DOI: 10.1016/j.theriogenology.2014.08.014
Singh, B., S.P.S. Ghuman, R.S. Cheema and A.K. Bansal. 2016. Melatonin implant induces estrus and alleviates oxidative stress in summer anestrus buffalo. Indian J. Anim. Reprod., 37(2): 28-32. Available on: https://acspublisher.com/journals/index.php/ijar/article/view/3653/3425
Singh, G., G.B. Singh and G.S. Dhaliwal. 1989. Studies on reproductive status of rural buffaloes in summer. Indian J. Anim. Reprod., 10: 151-153.
Vázquez, M.I., F. Forcada, A. Casao, J.A. Abecia, C. Sosa and I. Palacín. 2010. Undernutrition and exogenous melatonin can affect the in vitro developmental competence of ovine oocytes on a seasonal basis. Reprod. Domest. Anim., 45: 677-684. DOI: 10.1111/j.1439-0531.2008.01329.x
Viguie, C., A. Caraty, A. Locatelli and B. Malpaux. 1995. Regulation of luteinizing hormone-releasing hormone (LHRH) secretion by melatonin in the ewe. I. Simultaneous delayed increase in LHRH and luteinizing hormone pulsatile secretion. Biol. Reprod., 52(5): 1114-1120. DOI: 10.1095/biolreprod52.5.1114
Baruselli, P.S., N.A.T. Carvalho, L.U. Gimenes and G.A. Crepaldi. 2007. Fixed-time artificial insemination in buffalo. Ital. J. Anim. Sci., 6(2S): 107-118. DOI: 10.4081/ijas.2007.s2.107
D’Occhio, M.J., S.S. Ghuman, G. Neglia, G. Valle, P.S. Baruselli, L. Zicarelli, J.A. Visintin, M. Sarkar and G. Campanile. 2020. Exogenous and endogenous factors in seasonality of reproduction in buffalo: A review. Theriogenology, 150: 186-192. DOI: 10.1016/j.theriogenology.2020.01.044
Forcada, F., J.A. Abecia, J.A. Cebrián-Pérez, M.T. Muino-Blanco, J.A. Valares and I. Palacín. 2006. The effect of melatonin implants during the seasonal anestrus on embryo production after superovulation in aged high-prolificacy Rasa Aragonesa ewes. Theriogenology, 65(2): 356-365. DOI: 10.1016/j.theriogenology.2005.05.038
Forcada, F., J.A. Abecia, O. Zuniga and J.M. Lozano. 2002. Variation in the ability of melatonin implants inserted at two different times after the winter solstice to restore reproductive activity in reduced seasonality ewes. Aust. J. Agri. Res., 53(2): 167-173. DOI: 10.1071/AR00172
Ghuman, S.P.S., D. Dadarwal, M. Honparke, J. Singh and G.S. Dhaliwal. 2009. Production of polyclonal antiserum against progesterone for radioimmunoassay. Indian Vet. J., 86(9): 909-911.
Ghuman, S.P.S. and D.S. Dhami. 2017. Seasonal variation in AI and pregnancy rate in buffalo and improving their fertility status following application of FTAI during non-breeding season. Indian J. Anim. Reprod., 38(1): 4-8. Available on: https://acspublisher.com/journals/index.php/ijar/article/view/3556/3383
Ghuman, S.P.S., J. Singh, M. Honparkhe, D. Dadarwal, G.S. Dhaliwal and A.K. Jain. 2010. Induction of ovulation of ovulatory size non-ovulatory follicles and initiation of ovarian cyclicity in summer anoestrous buffalo heifers (Bubalus bubalis) using melatonin implants. Reprod. Domest. Anim., 45(4): 600-607. DOI: 10.1111/j.1439-0531.2008.01310.x
Habeeb, A.A., A.E. Gad and M.A. Atta. 2018. Temperature-Humidity Indices as indicators to heat stress of climatic conditions with relation to production and reproduction of farm animals. Int. J. Biotechnol. Recent. Adv., 1(1): 35-50. DOI: 10.18689/ijbr-1000107
Kavita, J.B. Phogat, A.K. Pandey, A.K. Balhara, S.P.S. Ghuman and P. Gunwant. 2018. Effects of melatonin supplementation prior to Ovsynch protocol on ovarian activity and conception rates in anestrous Murrah buffalo heifers during out of breeding season. Reprod. Biol., 18(2): 161-168. DOI: 10.1016/j.repbio.2018.03.001
Misztal, T., K. Romanowicz and B. Barcikowski. 2002. Effect of melatonin on daily LH secretion in intact and ovariectomized ewes during the breeding season. Anim. Reprod. Sci., 69(3-4): 187-198. DOI: 10.1016/s0378-4320(01)00194-4
Pandey, A.K., S.P.S. Ghuman, G.S. Dhaliwal, M. Honparkhe, J.B. Phogat and S. Kumar. 2018. Effects of preovulatory follicle size on estradiol concentrations, corpus luteum diameter, progesterone concentrations and subsequent pregnancy rate in buffalo cows (Bubalus bubalis). Theriogenology, 107: 57-62. DOI: 10.1016/j.theriogenology.2017.10.048
Pandey, A.K., P. Gunwant, N. Soni, Kavita, S. Kumar, A. Kumar, A. Magotra, I. Singh, J.B. Phogat, R.K. Sharma, Y. Bangar, S.P.S. Ghuman and S.S. Sahu. 2019. Genotype of MTNR1A gene regulates the conception rate following melatonin treatment in water buffalo. Theriogenology, 128: 1-7. DOI: 10.1016/j.theriogenology.2019.01.018
Ramadan, T.A. 2017. Role of melatonin in reproductive seasonality in buffaloes, p. 87-107. Theriogenology. DOI: 10.5772/intechopen.69549
Ramadan, T.A., R.K. Sharma, S.K. Phulia, A.K. Balhara, S.S. Ghuman and I. Singh. 2014. Effectiveness of melatonin and controlled internal drug release device treatment on reproductive performance of buffalo heifers during out-of-breeding season under tropical conditions. Theriogenology, 82(9): 1296-1302. DOI: 10.1016/j.theriogenology.2014.08.014
Singh, B., S.P.S. Ghuman, R.S. Cheema and A.K. Bansal. 2016. Melatonin implant induces estrus and alleviates oxidative stress in summer anestrus buffalo. Indian J. Anim. Reprod., 37(2): 28-32. Available on: https://acspublisher.com/journals/index.php/ijar/article/view/3653/3425
Singh, G., G.B. Singh and G.S. Dhaliwal. 1989. Studies on reproductive status of rural buffaloes in summer. Indian J. Anim. Reprod., 10: 151-153.
Vázquez, M.I., F. Forcada, A. Casao, J.A. Abecia, C. Sosa and I. Palacín. 2010. Undernutrition and exogenous melatonin can affect the in vitro developmental competence of ovine oocytes on a seasonal basis. Reprod. Domest. Anim., 45: 677-684. DOI: 10.1111/j.1439-0531.2008.01329.x
Viguie, C., A. Caraty, A. Locatelli and B. Malpaux. 1995. Regulation of luteinizing hormone-releasing hormone (LHRH) secretion by melatonin in the ewe. I. Simultaneous delayed increase in LHRH and luteinizing hormone pulsatile secretion. Biol. Reprod., 52(5): 1114-1120. DOI: 10.1095/biolreprod52.5.1114
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Published
2023-06-30
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Ghuman, S. S., Honparkhe, M., & Singh, B. (2023). Effect of melatonin implantation prior to ovsynch plus cidr protocol on subsequent ovarian activity and pregnancy rate in summer anestrous Murrah buffaloes. Buffalo Bulletin, 42(2), 263–269. https://doi.org/10.56825/bufbu.2023.4223586
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