Immunoreactive polypeptide profiles of Sarcocystis fusiformis using hyperimmune and naturally infected serum

Authors

  • Amit Singh Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India
  • Daya Shanker Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh, India
  • Amit Kumar Jaiswal Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh, India
  • Sanjeev Kumar Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh, India

DOI:

https://doi.org/10.56825/bufbu.2022.4123602

Keywords:

Bubalus bubalis, Buffaloes, Sarcocystis fusiformis, Polypeptides, SDS-PAGE, Western blot

Abstract

Immunoreactive polypeptide profiles study was made in the whole cyst lysate antigen of Sarcocystis fusiformis which was collected from buffaloes slaughtered at local slaughterhouse, Mathura, U.P. When whole cyst lysate antigen was subjected to SDS-PAGE, twelve major polypeptides (78, 66, 53, 50, 42, 39, 32, 29, 27, 24, 19 and 15kDa) were observed. On western blotting, out of twelve, six (32, 39, 42, 53, 66, 78kDa) and two polypeptides (53kDa and 66kDa) were found to be immunoreactive when probed with homologous hyperimmune and pooled known positive natural serum. No immunoreactivity was seen with pooled known negative natural serum. These two polypeptides (53kDa and 66kDa) bands can be explored for the development of sensitive and specific diagnostic assays.

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Author Biographies

Amit Singh, Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India



asingh.vet@gmail.com

Daya Shanker, Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh, India

Daya Shanker

Amit Kumar Jaiswal, Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh, India

Amit Kumar Jaiswal

Sanjeev Kumar, Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh, India

Sanjeev Kumar

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Published

2022-06-26

How to Cite

Singh, A., Shanker, D., Jaiswal, A. K., & Kumar, S. (2022). Immunoreactive polypeptide profiles of Sarcocystis fusiformis using hyperimmune and naturally infected serum. Buffalo Bulletin, 41(2), 329–335. https://doi.org/10.56825/bufbu.2022.4123602

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Original Article