Immunoreactive polypeptide profiles of Sarcocystis fusiformis using hyperimmune and naturally infected serum
DOI:
https://doi.org/10.56825/bufbu.2022.4123602Keywords:
Bubalus bubalis, Buffaloes, Sarcocystis fusiformis, Polypeptides, SDS-PAGE, Western blotAbstract
Immunoreactive polypeptide profiles study was made in the whole cyst lysate antigen of Sarcocystis fusiformis which was collected from buffaloes slaughtered at local slaughterhouse, Mathura, U.P. When whole cyst lysate antigen was subjected to SDS-PAGE, twelve major polypeptides (78, 66, 53, 50, 42, 39, 32, 29, 27, 24, 19 and 15kDa) were observed. On western blotting, out of twelve, six (32, 39, 42, 53, 66, 78kDa) and two polypeptides (53kDa and 66kDa) were found to be immunoreactive when probed with homologous hyperimmune and pooled known positive natural serum. No immunoreactivity was seen with pooled known negative natural serum. These two polypeptides (53kDa and 66kDa) bands can be explored for the development of sensitive and specific diagnostic assays.
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References
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Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature, 227: 680-685. DOI: 10.1038/227680A0
Mamatha, G.S., P.E. D’Souza and V.V.S. Suryanarayana. 2008. Serodiagnosis of bovine sarcocystosis by enzyme immune transfer blot (EITB) in naturally infected cattle. J. Vet. Parasitol., 22(2): 69-72.
Towbin, H., T. Staehelin and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. P. Natl. Acad. Sci. USA., 76(9): 4350-4354. DOI: 10.1073/pnas.76.9.4350
Bhatia, B.B. 2000. Textbook of Veterinary Protozoology. Indian Council of Agricultural Research - Krishi Anusandhan Bhavan, New Delhi, India. p. 236-255.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1-2): 248-252. DOI: 10.1016/0003-2697(76)90527-3
Dubey, J.P., C.A. Speer and R. Fayer.1989. Sarcocystosis of Animals and Man, CRC Press, Inc. Boca Raton, Florida, USA.
Fayer, R. and J.P. Dubey. 1986. Bovine sarcocystosis. Compendium on Continuing Education for the Practising Veterinarians, 8: 130-142.
Gasbarre, L.C., S. Pamela and R. Fayer. 1984. Humoral and cellular immune responses in cattle and sheep inoculated with Sarcocystis. Am. J. Vet. Res., 45(8): 1592-1596.
Granstrom, D.E., R.K. Ridley, Y. Baoan and L.J. Gershwin. 1990. Immunodominant proteins of Sarcocystis cruzi bradyzoites isolated from cattle affected or nonaffected with eosinophilic myositis. Am. J. Vet. Res, 51(7): 1151-1155.
Hamza. 2004. Identification and characterization of Sarcocystis fusiformis antigen before and after heat treatment. El-Minia Medical Bulletin, 15: 253-260.
Hettiarachchi, D.C. and R.P.V.J. Rajapakse. 2008. Antigenic analysis of bovine Sarcocystis spp. in Sri Lanka. Journal of National Science Foundation, Sri Lanka, 36(3): 239-244. Available on: http://dl.nsf.ac.lk/handle/1/6715
Khulbe, D.C., A. Kushwah, H.S. Kushwah and M.A. Quadri. 1989. Electrophoretic separation of the soluble proteins of different fractions of the sarcocyst of Sarcocystis fusiformis of buffalo (Bubalus bubalis). Vet. Parasitol., 32(3): 127-131. DOI: 10.1016/0304-4017(89)90113-1
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature, 227: 680-685. DOI: 10.1038/227680A0
Mamatha, G.S., P.E. D’Souza and V.V.S. Suryanarayana. 2008. Serodiagnosis of bovine sarcocystosis by enzyme immune transfer blot (EITB) in naturally infected cattle. J. Vet. Parasitol., 22(2): 69-72.
Towbin, H., T. Staehelin and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. P. Natl. Acad. Sci. USA., 76(9): 4350-4354. DOI: 10.1073/pnas.76.9.4350
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Published
2022-06-26
How to Cite
Singh, A., Shanker, D., Jaiswal, A. K., & Kumar, S. (2022). Immunoreactive polypeptide profiles of Sarcocystis fusiformis using hyperimmune and naturally infected serum. Buffalo Bulletin, 41(2), 329–335. https://doi.org/10.56825/bufbu.2022.4123602
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