Effect of CIDR protocol on conception rate in RFM affected and normally calved buffaloes


  • Mani Selvaraju Department of Veterinary Gynaecology and Obstetrics, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India
  • Kumar Ganesh Department of Veterinary Gynaecology and Obstetrics, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India




Bubalus bubalis, buffaloes, CIDR, RFM, oestrus induction, conception rate


Delayed re-establishment of ovarian cyclicity after parturition leads to increased inter-calving interval in buffaloes. This interval is further extended by the occurrence of post-partum disorders especially retained fetal membranes (RFM) which in turn causes heavy economic loss to the farming community. A total of 16 buffaloes at 45 to 60 days post-partum period including 8 buffaloes treated for retained fetal membranes (RFM) and 8 normally calved (NC) were selected and dewormed orally with Albendazole and administered orally with 50 g mineral mixture for 15 days after deworming. All the buffaloes at 60 to 75 days post-partum were inserted with CIDR intravaginally and left in situ for 9 days. All the buffaloes received an intra-muscular injection of 500 µg PGF2α at 24 h prior to CIDR withdrawal. At 48 (first AI) and 72 h (second AI) of CIDR removal, the buffaloes were artificially inseminated. Further, those buffaloes which failed to conceive and returned to oestrus (subsequent oestrus) following breeding at induced oestrus, were artificially inseminated twice at an interval of 24 h. Pregnancy diagnosis was done by rectal examination at 60 days post-insemination.
Retention rate of CIDR from insertion to withdrawal was 100%. The percentage of ovulatory response assessed by ultrasonography following oestrus induction in NC and RFM affected buffaloes was 100 and 87.50%. The first service, second service and overall conception rates were 25, 37.50 and 62.50 and 62.50, 25 and 87.50 in RFM and NC groups respectively.
In both the groups, the serum mean progesterone levels increased from the time of selection to the time of PGF2α injection but at first AI, the serum progesterone levels reached below 0.5 ng/ml and at 10 days post AI, there was an elevated progesterone levels in both the groups. The serum oestradiol levels increased from the time selection of animals to the time of first AI and then declined at 10 days post AI. The mean serum oestradiol levels were higher in NC buffaloes from the time of selection of animal to first AI in all the groups when compared to RFM affected buffaloes. It is concluded that oestrus induction with CIDR plus PGF2α with prior deworming and mineral mixture supplementation could be used in post-partum NC or RFM affected buffaloes to achieve maximum conception rate under field conditions.


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Andurkar, S.B. and S.B. Kadu. 1995. Induction of estrus and fertility with CIDR device and combination with PGF2α in non-cycling buffaloes. Indian Journal of Animal Reproduction, 16: 81-84.

Colazo, M.G., J.P. Kastelic, P.R. Whittaker, Q.A. Gavaga, R. Wilde and R.J. Mapletoft. 2004. Fertility in beef cattle given a new or previously used CIDR insert and estradiol, with or without progesterone. Anim. Reprod. Sci., 81(1-2): 25-34. DOI: 10.1016/j.anireprosci.2003.09.003

Duchens, M., M. Forsberg, L.E. Edquist, H. Gustafsson and H. Rodriguez-Marhnes. 1995. Effect of induced suprabasal progesterone levels around estrus on plasma concentrations of progesterone, estradiol-17beta and LH in heifers. Theriogenology, 42(7): 1159-1169. DOI: 10.1016/0093-691x(94)90864-8

El-Malky, O., M. Youssef, N. Abdel-Aziz and A. Abd El- Salaam. 2010. Postpartum performance of buffaloes treated with GnRH to overcome the impact of Placenta Retention. Journal of American Science, 6(5): 225-233. Available on: https://www.jofamericanscience.org/journals/am-sci/am0605/32_2580_am0605_225_233.pdf

El-Wishy, A.B. 2007. The postpartum buffalo: ii. Acyclicity and anestrus. Anim. Reprod. Sci., 97(3-4): 216-236. DOI: 10.1016/j.anireprosci.2006.03.003

Ghallab, R.S., F.M. Hussein and W.M.B. Noseir. 2016. Comparative efficiency of different cidr protocols for treatment of postpartum anestrous in Egyptian buffaloes. Alexandria Journal of Veterinary Sciences, 49(2): 149-156. DOI: 10.5455/ajvs.211044

Gohar, M.A., M.A. Elmetwally, A.M. Samy and M. Zaabel. 2018. Effect of oxytetracycline treatment on postpartum reproductive performance in dairy buffalo-cows with retained placenta in Egypt. Journal of Veterinary Healthcare, 1(3): 45-53. DOI: 10.14302/issn.2575-1212.jvhc-18-2146

Kimura, K., T.A. Reinhardt and J.P. Goff. 2006. Parturition and hypocalcemia blunts calcium signals in immune cells of dairy cattle. J. Dairy Sci., 89(7): 2588-2595. DOI: 10.3168/jds.S0022-0302(06)72335-9

Konyves, L., O. Szenci, V. Jurkovich, L. Tegzes, A. Tirian, N. Solymosi, G. Gyulay and E. Brydl. 2009. Risk assessment and consequences of retained placenta for uterine health, reproduction and milk yield in dairy cows. Acta Vet. Brno, 78(1): 163-172. DOI: 10.2754/avb200978010163

Murugavel, K., D. Antoine, M.S. Raju and F. Lopez-Gatius. 2009. The effect of addition of equine chorionic gonadotropin to a progesterone based estrous synchronization protocol in buffaloes (Bubalus bubalis) under tropical conditions. Theriogenology, 71(7): 1120-1126. DOI: 10.1016/j.theriogenology.2008.12.012

Nath, H.C., D.J. Dutta, A. Dutta and R.K. Biswas. 2003. Progesterone and estradiol profile in postpartum anestrus cow following Crestar and PMSG administration. Indian J. Anim. Sci., 73: 1102-1104.

Pandey, A.K., S.P. Shukla, S.K. Pandey and Y.K. Sharma. 2007. Haemato-biochemical profile in relation to normal parturition buffaloes and buffaloes with retained fetal membranes. Buffalo Bull., 26(2): 46-49. Available on: https://kukrdb.lib.ku.ac.th/journal/BuffaloBulletin/search_detail/result/286147

Pursely, J.R., M.O. Mee and M.C. Wiltbank. 1995. Synchronization of ovulation in dairy cows using PGF2alpha and GnRH. Theriogenology, 44(7): 915-923. DOI: 10.1016/0093-691x(95)00279-h

Rahman, M.S., A.S. Shohag, M.M. Kamal, N. Parveen and M. Shamsuddin. 2012. Application of ultrasonography to investigate postpartum anoestrus in water buffaloes. Reproductive and Developmental Biology, 36(2): 103-108.

Rajkumar, R. and S.K. Srivatsava. 2008. Serum thyroid hormones and macro minerals in cyclic and acyclic crossbred cows. Indian Vet. J., 85: 445-446.

Ravikumar, K. 2003. Synchronization of ovulation using ovsynchand ovsynch plus CIDR and fertility in postpartum anestrus buffaloes. M.V.Sc. Thesis, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.

Rhodes, F.M., S. McDougall, C.R. Burke, G.A. Verkerk and K.L. Macmillan. 2003. Treatment of cows with an extended postpartum anestrous interval. J. Dairy Sci., 86(6): 1876-1894. DOI: 10.3168/jds.S0022-0302(03)73775-8

Selvaraju, M., C. Veerapandian, D. Kathiresan, K. Kulasekar and C. Chandrahasan. 2011. Influence of hCG and synchromate-B treatment on pregnancy rates in repeat breeder cows. Indian Vet. J., 88(10): 100-101.

Sheldon, I.M. and H. Dobson. 2004. Postpartum uterine health in cattle. Anim. Reprod. Sci., 82-83: 295-306. DOI: 10.1016/j.anireprosci.2004.04.006

Sheldon, M.I., D.E. Noakes, A.N. Rycroft, D.U. Pfeiffer and H. Dobson. 2002. Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. Reproduction, 123(6): 837-845. DOI: 10.1530/rep.0.1230837

Singh, H. and T. Singh. 2006. Oestrus induction, plasma steroid hormone profiles and fertility response after CIDR and eCG treatment in acyclic Sahiwal cows. J. Anim. Sci., 19(11): 1566-1573. DOI: 10.5713/ajas.2006.1566

Singh, J., S.P.S. Ghuman D. Dadarwal, M. Honparkhe, G.S. Dhaliwal and A.K. Jain. 2010. Estimations of blood plasma metabolites following melatonin implants treatment for initiation of ovarian cyclicity in true anestrus buffalo heifers. Indian J. Anim. Sci., 80(3): 229-231.

Snedecor, G.M. and W.C. Cochran. 1994. Statistical Methods, 9th ed. Oxford and IBM Publishing Company, Mumbai, India.

Tregaskes, H., L.A. Guilbault and J.J. Dufour. 1994. Synchronization of ovarian follicular waves with a gonadotropin-releasing hormone agonist to increase the precision of estrus in cattle: A review. J. Anim. Sci., 73(10): 3141-3151. DOI: 10.2527/1995.73103141x

Zaabel, S.M., A.O. Hegab, A.E. Montasser and H. El-Sheikh. 2009. Reproductive performance of anestrous buffaloes treated with CIDR. Anim. Reprod., 6(3): 460-464.




How to Cite

Selvaraju, M., & Ganesh, K. (2024). Effect of CIDR protocol on conception rate in RFM affected and normally calved buffaloes. Buffalo Bulletin, 43(1), 37–44. https://doi.org/10.56825/bufbu.2024.4313683



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