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Present study estimated status of Johne’s disease in the buffalo population of Malwa region using goat based Indigenous plate ELISA test. Of 156 serum samples screened 41.0% (64) and 85.8% (134) were positive for MAP infection by Indigenous plate ELISA kit; condition (A), condition (B), respectively. Study showed that despite high slaughter rate, prevalence of Johne’s disease was high in native population of riverine buffaloes (Bubalus bubalis) and call for immediate control of disease. The present findings indicated that higher number of animals infected with JD may be responsible for reduced productivity or un-productivity and weight loss in buffaloes. Therefore systematic screening of buffaloes with indigenous sensitive and specific test for detection of Johne’s disease at certain time interval is required. So that spread of infection to other animals and herds can be reduced or avoided. The goat based ‘Indigenous ELISA kit’ was good herd screening test with high sensitivity and is recommended for the large scale screening of Johne’s disease in the domestic livestock population.