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In present investigation efficacy of AV/FRC/18 (Keetguard Liquid) a polyherbal ectoparasiticidal were assessed against mite infestation in buffalo calves. All the animals were infested with Sarcoptis spp. of mites. Percent efficacy of AV/FRC/18 against mite infestation was recorded and it was found that thrice a week application of 1:20 dilution results in 98% on day 7th post-treatment and maximum period of effectiveness was found 90% up to 30 days post treatment. Lesion scores were also recorded and it was found that on application of AV/FRC/18 thrice a week all the lesions were subsided on day 7th post treatment.