EFFECT OF UTERINE LAVAGE, LEVAMISOLE, PGF2α AND ITS COMBINATIONS ON PHYSICO-CHEMICAL PROPERTIES OF CERVICO-VAGINAL MUCUS AND RECOVERY RATE IN THE ENDOMETRIC BUFFALOES

Narendra Singh; Bhoopendra Singh; Rajesh Kumar

doi:10.56825/bufbu.2025.4445189

Authors

Narendra Singh Department of Veterinary Clinical Complex, R.R. College of Veterinary and Animal Science, Rajasthan, India
Bhoopendra Singh Department of Veterinary Gynaecology and Obstetrics, Colleges of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India
Rajesh Kumar Department of Veterinary Gynaecology and Obstetrics, Colleges of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

DOI:

https://doi.org/10.56825/bufbu.2025.4445189

Keywords:

Bubalus bubalis, buffaloes, endometritis, levamisole, uterine lavage, PGF2α

Abstract

This investigation aimed to assess the therapeutic efficacy of uterine lavage, levamisole, PGF₂α, and their combinations in managing 36 subclinical endometritic repeat breeder buffaloes at the Veterinary Clinical Complex, College of Veterinary and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, and the State Veterinary Hospital in the surrounding regions of Kumarganj. Thirty-six repeat breeder buffaloes were randomly and evenly allocated into six Groups A (positive control), B (uterine lavage), C (Levamisole), D (PGF₂α), E (uterine lavage plus Levamisole), and F (uterine lavage plus PGF₂α). The recovery rate in descending orders was noted as 83.33, 83.33, 66.67, 66.67, 50, and 33.33% in Groups F, E, D, C, B, and A, respectively. Moreover, the corresponding conception rates were 66.67, 66.67, 50.00, 50.00, 33.33, and 16.67% Groups F, E, D, C, B, and A, respectively. The recovery rate was significantly higher in Groups E and F; lower in Group B, and intermediate in Groups C and D, as compared to the control. Similar trends were also observed in conception rate. In conclusion, the uterine lavage, along with levamisol or PGF₂α, is equally effective in the treatment of endometritis in buffaloes.

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Author Biographies

Narendra Singh, Department of Veterinary Clinical Complex, R.R. College of Veterinary and Animal Science, Rajasthan, India

Narendra Singh

Bhoopendra Singh, Department of Veterinary Gynaecology and Obstetrics, Colleges of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

Bhoopendra Singh

drrajesh25@gmail.com

Rajesh Kumar, Department of Veterinary Gynaecology and Obstetrics, Colleges of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

Department of Veterinary Gynaecology and Obstetrics, Colleges of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

References

Arjunrao, T.G. 2017. Efficacy of neem extract, lugol’s iodine and E.coli LPS for the treatment of endometritis in cows and buffaloes. M.V.Sc. Thesis, Maharashtra Animal and Fishery Sciences University, Nagpur, India.

Bedewy, R.B. and M.A. Rahaway. 2019. Comparative study for detection of subclinical endometritis in local cows. Adv. Anim. Vet. Sci., 7(4): 289-294. DOI: https://doi.org/10.17582/journal.aavs/2019/7.4.289.294

Biswal, S., S. Das, D.N. Mohanty and D. Jena. 2014. Effect of systemic and local immunomodulation therapies on conception rate in endometritic cows. Indian Journal of Veterinary Sciences and Biotechnology, 10(2): 1-4.

Brinsko, S.P., T.L. Blanchard, D.D. Varner, J. Schumacher, C.C. Love, K. Hinrichs and D.L. Hartman. 2011. Endometritis. Manual of Equine Reproduction, 3rd ed. Elsevier Health Sciences, Missouri, USA. p. 73-84.

Burnner, C.J. and C.C. Muscoplat. 1980. Immunomodulatory effects of levamisole. J. Am. Vet. Med. Assoc., 176: 1159-1162.

Butani, M.G, R. Kumar, A.J. Dhami, F.S. Kavani and A. Killedar. 2008. Incidence of major infertility problems in crossbred cows and buffaloes under field conditions. Indian Journal of Field Veterinarians, 4(2): 1-4.

Butani, M.G., A.J. Dhami, R. Kumar, N.D. Hirani, V.P. Ramani and K.P. Patel. 2009. Influence of hormonal and antibiotics therapy on fertility and trace minerals profile in repeat breeding buffaloes. Indian Journal of Field Veterinarians, 4(3): 12-16.

Butani, M.G., A.J. Dhami, R.G. Shah, N.P. Sarvaiya and A. Killedar. 2016. Management of repeat breeding in buffaloes under field conditions Using hormonal and antibacterial therapies. Buffalo Bull., 35(1): 83-91. Available on: https://kukrdb.lib.ku.ac.th/journal/BuffaloBulletin/search_detail/result/331434

Dabas, Y.P.S. and S.N. Maurya. 1988. A field method for collection of bovine cervical mucus for microbiological studies. Indian J. Anim. Reprod., 9(2): 138-139.

Deori, S. 2002. Use of bacterial modulins in treatment of endometritis cows, Thesis, M.V.Sc. Indian Veterinary Research Institute, Izatnagar, IndiaHafez, E.S.E. and B. Hafez. 2000. Reproduction in Farm Animals, 7th ed., Lea and Febiger, Philadelphia, USA. p. 41-46.

Krishnan, B.B., H. Kumar, S. Mehrotra, S.K. Singh, T.K. Goswami, F.A. Khan, M.K. Patra and R. Islam. 2015. Effect of leukotriene B4 and oyster glycogen in resolving subclinical endometritis in repeat breeding crossbred cows. Indian J. Anim. Res., 49(2): 218-222. DOI: https://doi.org/10.5958/0976-0555.2015.00112.0

Kumar R., M.G. Butani, A.J. Dhami, F.S. Kavani, C.D. Bhong and M.N. Brahmbhatt 2008. Isolation and antibiogram of genital microflora from repeat breeding cows and buffaloes. Indian Journal of Field Veterinarians, 4(2): 5-9. DOI: https://doi.org/10.13140/RG.2.2.11593.57447

Kumar, R., A.J. Dhami, M.G. Butani, F.S. Kavani, N.P. Sarvaiya, and A. Killledar. 2010. Management M.G. of repeat breeding under field condition using hormonal and non hormonal drugs in cows. Indian Journal of Animal Reproduction, 31(1): 30-32.

Kumar, R., M.G. Butani, A.J. Dhami, F.S. Kavani, and R.G. Shah. 2009. Effect of different therapies on fertility and serum progesterone, metabolites and minerals profile in repeat breeding crossbred cows. Indian Journal of Field Veterinarians, 5(2): 1-8.

Kumar, R., M.G. Butani, F.S. Kavani, and A.J. Dhami. 2020. Hormonal interventions to augment fertility and its effect on blood biochemical profile in crossbred cows. Haya: The Saudi Journal of Life Sciences, 5(9): 176-181. Available on: https://saudijournals.com/media/articles/SJLS_59_176-181_c_q5ov0tS.pdf

Kumar, S., A. Bhardwaz, A.K. Srivastava, M. Rao and N. Kumar. 2015. White side test - A field test on the cervical mucus of cows for diagnosis of endometritis. Intas Polivet, 16(2): 207-213.

Lai, S.K., Y.Y. Wang, D. Wirtz and J. Hanes. 2009. Micro- and macrorheology of mucus. Adv. Drug Deliv. Rev., 61(2): 86-100. DOI: https://doi.org/10.1016/j.addr.2008.09.012

Lim, H.J., S. Jun-Kyu, Y. Ho-Beak, B. Kwang-Soo, K. Tae-il, J. Yeon-Sub and K. Eung-Gi. 2014. Physical properties of estrus mucus in relation to conception rates in dairy cattle. Journal of Embryo Transfer, 29(2): 157-161. DOI: https://doi.org/10.12750/JET.2014.29.2.157

Lindell, J.O. and H. Kindahl. 1983. Exogenous prostaglandin F2 alpha promotes uterine involution in the cow. Acta. Vet. Scand., 24(3): 269-274. DOI: https://doi.org/10.1186/BF03546730

Liu, I. and M. Troedsson. 2008. The diagnosis and treatment of endometritis in the mare: yesterday and today. Theriogenology, 70(3): 415-420. DOI: https://doi.org/10.1016/j.theriogenology.2008.05.040

Markusfeld, O. 1984. Factors responsible for post parturient metritis in dairy cattle. Vet. Rec., 114(22): 539-542. DOI: https://doi.org/10.1136/vr.114.22.539

Ohtani, S., K. Okuda, K. Nishimura and S. Mohri. 1993. Histological changes in bovine endometrium during the estrous cycle. Theriogenology, 39(5): 1033-1042. DOI: https://doi.org/10.1016/0093-691X(93)90004-O

Paisley, L.G., W.D. Mickelsen and P.B. Anderson. 1986. Mechanisms and therapy for retained fetal membranes and uterine infections of cows. A review. Theriogenology, 25(3): 353-381. DOI: https://doi.org/10.1016/0093-691x(86)90045-2

Palanisamy, M., R.E. Napolean, M. Selvaraju, K. Krishnakumar, G.A. Balasubramanian, S. Malmarugan and S. Manokaran. 2014. Nature of genital discharge and pH of cervical mucus and uterine flushing before and after treatment in endometritis affected cows. International Journal of Livestock Research, 4(7): 19-24. DOI: https://doi.org/10.5455/ijlr.20141006071857

Parikh, S.S., F.S. Kavani, K.H. Parmar, T.K. Patbandha, V.K. Singh, A.R. Ahlawat and R. Kumar. 2022. Diagnostic and therapeutic management of subclinical endometritis in dairy bovine: A review. Animal Reproduction Update, 2(2): 1-11. DOI: https://doi.org/10.48165/aru.2022.2.2.1

Pateria, A.K. and C.V.S. Rawal. 1990. White side test for subclinical metritis in buffaloes. Indian Journal of Animal Reproduction, 11: 142-144.

Reddy, N.C.S., K.V. Bramhaiah, K.S. Naidu, A.J. Babu and R.V.S. Kumar. 2012. Effect of uterine lavage on bacterial count and conception rate in repeat breeder crossbred cows. Indian Journal of Animal Reproduction, 33(1): 59-62.

Rutllant, J., M. Lopez-Bejar and F. Lopez-Gatius. 2005. Ultrastructural and rheological properties of bovine vaginal fluid and its relation to sperm motility and fertilization: A review. Reprod. Domest. Anim., 40(2): 79-86. DOI: https://doi.org/10.1111/j.1439-0531.2004.00510.x

Sahadev, A., M. Devara, T.G. Honnappa and V.C. Murthy. 2007. Effect of immunomodulators on biochemical attributes of cows with endometritis. Indian Journal of Animal Reproduction, 28(1): 66-68.

Saini, P.S., A.S. Nanda, A.S. Grewal and J. Singh. 1999. Uterine defense modulation for the treatment of repeat breeding due to infectious endometritis in bovines. Indian J. Anim. Sci., 69(5): 307-309.

Salphale, G.B., M.M. Kadu, M. Fasihddun and M.S. Kadu. 1993. Study of some physical properties of estrus cervical mucus in synchronized animals and repeat breeder crossbred cows with reference to fertility. Indian Journal of Animal Reproduction, 14(2): 77-78.

Sarkar, P., H. Kumar and M.K. Patra. 2015. Leucocytes enriched autologous plasma for the management of bovine endometritis. Intas Polivet, 16(2): 214-217.

Sarkar, P., H. Kumar, M. Rawat, V.P. Varshney, T.K. Goswami, M.C. Yadav and S.K. Srivastava. 2006. Effect of administration of garlic extract and PGF2α on hormonal changes and recovery in endometritis cows. Asian-Austral. J. Anim. Sci., 19(7): 964-969. DOI: https://doi.org/10.5713/ajas.2006.964

Selvaraj, P., H. Kumar and S. Mahmood. 2002. Physico-enzymatic characteristics of cervical mucus with reference to fertility in repeat breeding cows. Indian Journal of Animal Reproduction, 23(1): 54-56.

Sheehan, J.K., M. Kesimer and R. Pickles. 2006. Innate immunity and mucus structure and function. Novartis Foundation Symposia, 279: 155-166.

Sheldon, I.M., G.S. Lewis, S. LeBlanc and R.O. Gilbert. 2006. Defining post partum uterine disease in cattle. Theriogenology, 65(8): 1516-1530. DOI: https://doi.org/10.1016/j.theriogenology.2005.08.021

Singh, P.P., N. Pandey, Bhavna and R. Agrawal. 2017. Clinical and biochemical studies on endometritic repeat breeding cows following treatment with levamisole. Haryana Veterinarian, 56(1): 55-57. Available on: https://www.luvas.edu.in/haryana-veterinarian/download/harvet2017/14.pdf

Singh, R. 2014. Comparative study on immunomodulatory and therapeutic efficacy of homeopathic and allopathic medicines in crossbred cows. M.V.Sc. Thesis, G.B. Pant University of Agriculture and Technology, Pantnagar, India.

Snedecor, G.W. and W.G. Cochran. 1989. Statistical Methods, 8th ed. Iowa State University Press, Ames, Iowa, USA.

Sood, P., S. Verma, R.C. Katoch, M. Singh and N.K. Vaishishta. 2003. Impact of uterine microbial panorama on the single injection of PGF2α in cows with clinical endometritis. Indian J. Anim. Sci., 73(1): 68-69.

Swain, P.K., D.N. Mohanty, S. Das, A.K. Barik, P.C. Mishra, A.K. Tripathi and T.K. Palai. 2011. Immunomodulation effect of levamisole and immulite on uterine microbial picture in repeat breeding cows. Indian Journal of Animal Reproduction, 32(3): 68-70.

Tsiligianni, T., A. Karagiannidis, P. Brikas and P. Saratsis. 2001. Chemical properties of bovine cervical mucus during normal estrus and estrus induced by progesterone and/or PGF2α. Theriogenology, 56(1): 41-50. DOI: https://doi.org/10.1016/S0093-691X(01)00541-6

Tsiligianni, T., A. Karagiannidis, P. Saratsis and P. Brikas. 2003. Enzyme activity in bovine cervical mucus during spontaneous and induced estrus. Can. J. Vet. Res., 67(3): 189-193.