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The present study was undertaken to study the effect of ovsynch protocol in different hormonal combinations on twenty four anestrous Murrah buffaloes (Bubalus bubalis), randomly divided into four equal groups. Group I was kept as untreated control. Group II received Ovsynch protocol alone where as Ovsynch + CIDR and Ovsynch + Hydroxy-progesterone were administered in group III and IV, respectively. In response to the Ovsynch treatment in different combinations 83%, 83% and 66% animals expressed estrus out of which 100%, 80% and 100% animals ovulated in Groups II, III and IV, respectively. Out of animals that expressed estrus, 60%, 20% and 0% in Groups II, Group III and Group IV, respectively were confirmed as pregnant using ultrasonography 30 days post A.I. Cost effectiveness per pregnancy in Group II and Group III was rupees 1385 and 8013, respectively. From the present investigation it can be concluded that Ovsynch alone is capable of inducing cyclicity and obtaining satisfactory conception rates in true anestrous buffaloes.