EFFECT OF BRADYKININ ON FREEZABILITY OF ABATTOIR  DERIVED MURRAH BULL EPIDIDYMAL SPERMATOZOA

Kumar Ashutosh; Sushant Srivastava; Rajesh Kumar; Pramod Kumar

doi:10.56825/bufbu.2025.4444303

Authors

Kumar Ashutosh Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India
Sushant Srivastava Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India
Rajesh Kumar Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India
Pramod Kumar Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

DOI:

https://doi.org/10.56825/bufbu.2025.4444303

Keywords:

Bubalus bubalis, buffaloes, additive, bradykinin, dilutors, epididymal spermatozoa, Murrah bull

Abstract

The research was performed on thirty-six (36) pairs of buffalo bull tests obtained from an abattoir. This study aimed to assess the impact of bradykinin on seminal characteristics, including progressive motility, viability, abnormalities, and HOS reactivity, in post-dilution, pre-freeze, and post-thaw semen obtained from the epididymis. Seminal parameters were evaluated in relation to skim milk, tris, citrate, skim milk combined with bradykinin, tris combined with bradykinin, and citrate combined with bradykinin. Notable discrepancies were seen in all metrics, specifically post-thaw motility, viability, HOS reactivity, and sperm abnormalities. In conclusion, the inclusion of bradykinin in the dilutor markedly enhances post-thaw progressive motility, viability, and HOS responsiveness relative to the Control group. Bradykinin can be utilized to enhance the quality of frozen-thawed semen from Murrah bulls.

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Author Biographies

Kumar Ashutosh, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

Kumar Ashutosh

Sushant Srivastava, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

Sushant Srivastava
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

Rajesh Kumar, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

Rajesh Kumar*
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

Pramod Kumar, Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

Pramod Kumar
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

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