Morphometric study of slaughtered Murrah buffalo bull testis and its correlation with seminal attributes of epididymal semen
DOI:
https://doi.org/10.56825/bufbu.2022.4143718Keywords:
Bubalus bubalis, buffaloes, morphometry, Murrah buffalo bull, seminal attributes, testisAbstract
Present study was conducted to study the morphometric characteristics of slaughtered Murrah buffalo bull testis and its correlation with seminal attributes of epididymal semen. Total 56 pair of slaughtered Murrah buffalo bull testicles were procured from Allana, slaughterhouse, Arrora, and Mass agro food Pvt. Ltd. Unnao Uttar Pradesh, India; maintained at Deep Frozen Semen Laboratory, College of Veterinary science, and animal Husbadry, Ayodhya, Uttar Pradesh, India. Various morphometric parameters of testes and epididymis were analyzed and their correlations with attributes of epididymal semen were evaluated. No significance difference in seminal attributes between 6 h and 12 h after collection of testicles is clearly indicated that semen can be collect up to 12 h after death of animal without any significance loss in quality of semen in Murrah buffalo bull. Significant strong positive correlation among important characteristics (motility, livability and HOS positive sperm) suggests that these test may be used as one of the test for predicting fertility of epididymal semen Murrah buffalo bull.
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Bitto, I.I. and M.I. Okpale. 2006. Sperm production rate, Gonadal and extragonadal sperm reserves in slaughtered White Fulani (Bunaji) bulls in a lowland tropical environment. Nigerian Journal of Animal Production, 33(2): 300-307. DOI: 10.51791/njap.v33i2.942
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Bruemmer, J.E., H. Reger, G. Zibinski and E.L. Squires. 2002. Effect of storage at 5°C on the motility and cryopreservation of stallion epididymal spermatozoa. Theriogenology, 58(2): 405-407. DOI: 10.1016/S0093-691X(02)00838-5
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Ansari, M.S., B.A. Rakha and S. Akhter. 2011. Effect of L-cysteine in extender on post thaw quality of Sahiwal bull semen. Anim. Sci. P., 29(3): 197-203. Available on: https://www.igbzpan.pl/uploaded
Bansal, N., V. Uppal, Anuradha and K.S. Roy. 2003. Testicular and epididymal biometry of adult donkeys (Equus asinus). Centaur, 20: 33-35.
Bartels P., Y. Friedmann, K. Lubbe, D. Mortimer, L.A. Rasmussen and R.A. Godke. 2001. The live birth of an eland (Taurotragus oryx) calf following estrous synchronization and artificial insemination using frozen thawed epididymal sperm. Theriogenology, 55: 381.
Bartels, P., K. Lubbe, R.L. Smith and R.A. Godke. 1999. Morphological changes of caudal epididymal spermatozoa of African buffalo (Syncerus caffer) after storage at 6°C. Theriogenology, 51(1): 279. DOI: 10.1016/S0093-691X(99)91838-1
Bitto, I.I. and M.I. Okpale. 2006. Sperm production rate, Gonadal and extragonadal sperm reserves in slaughtered White Fulani (Bunaji) bulls in a lowland tropical environment. Nigerian Journal of Animal Production, 33(2): 300-307. DOI: 10.51791/njap.v33i2.942
Braun, J., M. Sakai, S. Hochi, and N. Oguri. 1994. Preservation of ejaculated and epididymal stallion spermatozoa by cooling and freezing. Theriogenology, 41(4): 809-818. DOI: 10.1016/0093-691x(94)90497-7
Bruemmer, J.E., H. Reger, G. Zibinski and E.L. Squires. 2002. Effect of storage at 5°C on the motility and cryopreservation of stallion epididymal spermatozoa. Theriogenology, 58(2): 405-407. DOI: 10.1016/S0093-691X(02)00838-5
Campbell, R.G., J.L. Hancock and L. Rothschild. 1953. Counting live and dead bull spermatozoa. J. Exp. Biol., 30(1): 30-44. DOI: 10.1242/jeb.30.1.44
Carr, D.W. and T.S. Acott. 1984. Inhibition of bovine spermatozoa by caudal epididymal fluid. I. Study of sperm motility quiescence factor. Biol. Reprod., 30(4): 913-925. DOI: 10.1095/biolreprod30.4.913
Deibel, F.C., J.E. Smith, B.G. Crabo and E.F. Graham. 1976. Evaluation of six assays of semen quality by means of their accuracy, precision and sensitivity in separating known induced levels of damage, p. 888-891. In Proceedings of the 8th International Congress on Animal Reproduction and Artificial Insemination, Cracow, Poland.
Dong, Q., S.E. Rodenburg, C. Huang and C.A. Vandevoort. 2008. Cryopreservation of rhesus monkey (Macaca mulatta) epididymal spermatozoa before and after refrigerated storage. J. Androl., 29: 283-292. DOI: 10.2164/jandrol.107.003921
Fields, M.J., W.C. Burns and A.C. Warnick. 1979. Age, season and breed effects on testicular volume and semen traits in young beef bulls. J. Anim. Sci., 48(6): 1299-1340. DOI: 10.2527/jas1979.4861299x
Hafez, E.S.E. and B. Hafez. 2000. Reproduction in Farm Animals, 7th ed. Lippincott Williams and Wilkins, Baltimore, USA.
Hirao, K. 1975. A multiple regression analysis on six measurements of bovine semen characteristics for estimating fertility. Int. J. Fetil., 20(4): 204-208.
Hori, T., M. Ichikawa, E. Kawakami and T. Tsutsui. 2004. Artificial insemination of frozen epididymal sperm in beagle dogs. J. Vet. Med. Sci., 66(1): 37-41. DOI: 10.1292/jvms.66.37
Hori, T., K. Hagiuda, S. Endo, A. Hayama, E. Kawakami and T. Tsutsui. 2005. Unilateral intrauterine insemination with cryopreserved caudal epididymal sperm recovered from refrigerated canine epididymides. J. Vet. Med. Sci., 67(11): 1141-1147. DOI: 10.1292/jvms.67.1141
Igboeli, G. and R.H. Foote. 1968. Maturation changes in bull epididymal spermatozoa. J. Dairy Sci., 51(10): 1703-1705. DOI: 10.3168/jds.S0022-0302(68)87257-1
James, A.N., H. Green, S. Hoffman, A.M. Landry, D. Paccamonti and R.A. Godke. 2002. Preservation of equine sperm stored in the epididymis at 4oC for 24, 48, 72 and 96 hours. Theriogenology, 58(2): 401-404. DOI: 10.1016/S0093-691X(02)00883-X
Jeyendran, R.S., H.H. Van Der Van, B. Perez-Palaez, G.M. Crabo and L.J.D. Zaneveld. 1994. Development of an essay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70(1): 219-228. DOI: 10.1530/jrf.0.0700219
Joshi, A., A.K. Mathur, S.M.K. Naqvi and J.P. Mittal. 2006. Influence of osmolality of complete semen extender on motion characteristics of frozen-thawed ram spermatozoa. Asian Austral. J. Anim. Sci., 19(12): 1716-1721. DOI: 10.5713/ajas.2006.1716
Julie, A.C., M. Scott, F. Kelly, T.H. Justin, D. Lisa and L.F. Mei. 2004. A comparison of electroinjaculation and epididyma sperm collection technique in stallions. Canadian Vet. J., 45(1): 35-41.
Kikuchi, K., J. Nagai, N. Kashiwazaki, H. Ikeda, J. Noguchi, A. Shimada, E. Soloy and H. Kaneko. 1998. Cryopreservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4oC. Theriogenology, 50(4): 615-623. DOI: 10.1016/s0093-691x(98)00166-6
Kumar, S. and S. Srivastava. 2017. Testicular biometry and its correlation with body weight and semen output in Murrah bull. Buffalo Bull., 36(1): 105-113. Available on: https://kukrdb.lib.ku.ac.th/journal/BuffaloBulletin/search_detail/result/366131
Linford, E., F.A. Glover, C. Bishop and D.L. Stewart. 1976. The relationship between semen evaluation methods and fertility in the bull. J. Reprod. Fertil., 47(2): 283-291. DOI: 10.1530/jrf.0.0470283
Luz, P.A.C., P.R.S. Santos, S. Andrighetto, A.M. Jorge and A.C.A. Neto. 2013. The correlation between age, body weight and testicular parameters in Murrah buffalo bulls raised in Brazil. J. Reprod. Develop., 59(1): 14-17. DOI: 10.1262/jrd.2012-021
Martinez-Pastor, F., C. Guerra, M. Kaabi, A.R. Diaz, E. Anel, P. Herraez, P. de Paz and L. Anel. 2005. Decay of sperm obtained from epididymis of wild ruminents depending on postmortem time. Theriogenology, 63(1): 24-40. DOI: 10.1016/j.theriogenology.2004.03.003
Martins, C.F., R. Rumpf, D.C. Pereira and M.N. Dode. 2007. Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in vitro embryo production. Anim. Reprod. Sci., 101(3-4): 326-331. DOI: 10.1016/j.anireprosci.2007.01.018
Nichi, M., T. Rijsselare, A. Van Soom, J.B.P. De Clercq, I.G.F. Goovaerts, V.H. Barnabe and P.E.J. Bols. 2007. Effect of bull epididymis storage conditions on cryopreserved epididymal sperm in vitro fertility and lipid peroxidation status. In The 33rd Annual International Embryo Transfer Society, Kyoto, Japan. Reprod. Fert. Develop., 19(1): 126. DOI: 10.1071/RDv19n1Ab15
Osinowo, O.A. 2006. Introduction to Animal Production, 1st ed. Sophie Academic, Abeokuta, Nigeria.
Osman, A.M and E.A. El-Azab. 1974. Gonadal and epididymal sperm reserves in the camel, Camelus Dromedarius. J. Reprod. Fertill., 38: 425-430. DOI: 10.1530/jrf.0.0380425
Pant, H.C., A.K. Mittal, R. Kasiraj, J.H. Prabhakar and A.K. Misra. 2002. Abnormal detached heads: A characteristic morphological abnormality in spermatozoa of Holstein Friesian x Sahiwal crossbred bulls. Indian J. Anim. Sci., 72(4): 316-318.
Pant, H.C., R.K. Sharma and S.H. Patel. 2003. Testicular development and its relationship to semen production in Murrah buffalo bulls. Theriogenology, 60(1): 27-34. DOI: 10.1016/s0093-691x(02)01037-3
Paulenz, H., I.S. Grevle, A. Tverdal, P.O. Hofmo and K.A. Berg. 1995. Precision of the coulter (R) counter for routine assessment of boar-sperm concentration in comparison with the hemocytometer and spectrophotometer. Reprod. Domest. Anim., 30(3): 107-111. DOI: 10.1111/j.1439-0531.1995.tb00614.x
Ponglowhapan, S., K. Chatdarong, S. Sirivaidyapong and C. Lohachit. 2006. Freezing of epididymal spermatozoa from dogs after cool storage for 2 or 4 days. Theriogenology, 66(6-7): 1633-1636. DOI: 10.1016/j.theriogenology.2006.01.032
Rizal, M., M.R. Toelihere, T.L. Yusuf, B. Purwantara and P. Situmorang. 2003. The quality of freeze-thawed Garut ram sperm in different concentration of glycerol. JITV, 7: 194-199.
Saacke, R.G. 1990. What is Abnormal? And is abnormal dependent upon the animal?, p. 67-73. In Proceeding of the NAAB 13th Technical Conference on AI and Reprod, Milwaukee, Wisconsin, USA.
Salisbury, G.W, N.K. Vandemark and J.R. Lodge. 1985. Semen Evaluation Physiology of Reproduction and Artificial Insemination of Cattle, 2nd ed. CBS Publishers and Distributors, Shadra, Delhi, India.
Saurabh, S. Srivastava, A. Kumar, S. Verma, P. Sharma and V. Gautam. 2018. Study of testicular biometry and its correlation with epididymal seminal attribute in buffalo bulls. The Pharma Innovation Journal, 7(6): 251-256. Available on: https://www.thepharmajournal.com/archives/2018/vol7issue6/PartD/7-6-75-402.pdf
Sengupta, B.P. and S.L. Bhela. 1988. Current status of male fertility research in buffaloes. In The Proceedings of 2nd World Buffalo Congress, New Delhi, India. p. 63.
Singh, L.P., H.M. Harshan and M.R. Ansari. 2007. Effect of egg yolk and seminal plasma heparin binding protein interac-tion on the freezability of buffalo cauda epididymal sper-matozoa. Anim. Reprod. Sci., 99(3-4): 395-400. DOI: 10.1016/j.anireprosci.2006.08.018
Siqueira, J.B., E. Oba, R.O. Pinho, E.F. Castilho, R.F. Bittencourt, S.S. Assaf1 and J.D. Guimarães. 2007. Gonadic sperm reserve in buffaloes created under tropical conditions. Ital. J. Anim. Sci., 6(2): 615-618. DOI: 10.4081/ijas.2007.s2.615
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2022-12-29
How to Cite
Kumar, H., Srivastava, S., & Kumar, R. (2022). Morphometric study of slaughtered Murrah buffalo bull testis and its correlation with seminal attributes of epididymal semen. Buffalo Bulletin, 41(4), 601–613. https://doi.org/10.56825/bufbu.2022.4143718
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