Testicular biometry and certain aspects of epididymal semen of abattoir derived Murrah bull testes

Authors

  • Kumar Ashotosh Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India
  • Sushant Srivastava Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India
  • Rajesh Kumar Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India
  • Pramod Kumar Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh, India

DOI:

https://doi.org/10.56825/bufbu.2023.4244312

Keywords:

Bubalus bubalis , buffaloes, biometry, Murrah buffalo bull, seminal attributes, testis

Abstract

The aim of this study to assess the biometric parameters of abattoir derived testicles of Murrah buffalo bull. Thirty-six pair of Murrah buffalo bull testicles were collected immediately after slaughter from different abattoir, packed in ice chest (4oC) and transferred to DFS Laboratory, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Ayodhya, UP, India. The tests were processed within six hours of collection. Various biometric parameters testes (testicular length, circumference, diameter, volume, and weight) epididymis (weight, length and width of caput and corpus as well as length and circumference of cauda) was measured. The epididymal semen was harvested and measured; Volume (0.43±0.02 to 0.52±0.05 ml), concentration (1708±105.2 to 1958±126.8 million/ml) and total sperm output (804.2±30.73 to 931.7±103.2 million) was estimated. All biometric parameters were significantly higher in left testicles than those of right contemporaries. Present findings suggest that aforementioned biometric parameters can be used to judge the normality of testis, furthermore, epididymal semen can be harvested from meritorious Murrah buffalo bulls with sudden or unexpected death.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Ali, K.M., N. Ahmad, N. Akhtar, S. Ali, M. Ahmad and M. Younis. 2011. Ultrasound imaging of testes and epididymis of normal and infertile breeding bulls. Pak. Vet. J., 31(4): 345-350.

AL-Sahaf, M.M. and N.S. Ibrahim. 2012. Monthly changes in testes and epididymis measurements with some semen characteristics of tail epididymis for Iraqi buffalo. Iraqi Journal of Veterinary Medicine, 36(2): 204-208. DOI: 10.30539/iraqijvm.v36i2.497

Baldaniya, R.V., C.M. Patel, N.F. Chaudhary, L.C. Modi, L.M. Chaudhary, N.S. Dangar and G.M. Pandya. 2020. Study of testicular biometry and its correlation with cauda epididymal buck seminal attributes. Haryana Veterinarian, 59(S1): 61-64. Available on: https://www.luvas.edu.in/haryana-veterinarian/download/harvet2020-march/15.pdf

Bartels, P., K. Lubbe, R.L. Smith and R.A. Godke. 1999. Morphological changes of caudal epididymal spermatozoa of African buffalo (Syncerus caffer) after storage at 6oC. Theriogenology, 51: 279.

Berndtson, W.E. 2014. Sperm production and its harvest, p. 15-21. In Chenoweth, P.J. and S.P. Lorton (eds.) Animal Andrology Theories and Applications. CABI, Wallingford, Oxfordshire, UK.

Chima, M.U., A.H. Abu, M.K. Dawuda, A.I. Kisani and T. Ahemen. 2017. Effect of storage time on cauda epididymal sperm parameters of Nigerian local dogs. Open Journal of Veterinary Medicine, 7: 151-161.

Dong, Q., S.E. Rodenburg, C. Huang and C.A. Vandevoort. 2008. Cryopreservation of rhesus monkey (Macaca mulatta) epididymal spermatozoa before and after refrigerated storage. J. Androl., 29(3), 283-292. DOI: 10.2164/jandrol.107.003921

El-Khawaga, A.R.M., M.M.M. Kandiel, G.A. Sosa, M.E.A. Abou El-Roos, A.E. Abdel-Ghaffar and A.E.S.I. El-Azab. 2012. Ultrasound imaging of the testes and accessory sex glands in buffalo bulls treated with gonadotrophic releasing hormone. Journal of Reproduction and Infertility, 3(1): 8-16. DOI: 10.5829/idosi.jri.2012.3.1.63186

Hafez, E.S.E. and B. Hafez. 2000. Reproduction in Farm Animals, 7th ed. Baltimore: Lippincott Williams and Wilkins, Philadelphia, USA.

Hori, T., M. Ichikawa, E. Kawakami and T. Tsutsui. 2004. Artificial insemination of frozen epididymal sperm in beagle dogs. J. Vet. Med. Sci., 66(1): 37-41. DOI: 10.1292/jvms.66.37

Hori, T., K. Hagiuda, S. Endo, A. Hayama, E. Kawakami and T. Tsutsui. 2005. Unilateral intrauterine insemination with cryopreserved caudal epididymal sperm recovered from refrigerated canine epididymides. J. Vet. Med. Sci., 67(11): 1141-1147. DOI: 10.1292/jvms.67.1141

Kang, S., U. Kim, M. Jeon, M. Lee and S. Cho. 2018. Comparison of spermatozoa recovery methods on cauda epididymal sperm of Hanwoo bulls. Journal of Embryo Transfer, 33(4): 321-326. DOI: 10.12750/JET.2018.33.4.321

Karagiannidis, A., S. Varsakeli and G. Karatzas. 2000. Characteristics and seasonal variations in the semen of Alpine, Saanen and Damascus goat bucks born and raised in Greece. Theriogenology, 53(6): 1285-1293. DOI: 10.1016/S0093-691X(00)00272-7

Mahmood, S., A. Kumar, R. Singh, M. Sarkar, G. Singh, M.R. Verma and G. Kumar. 2018. Scrotal circumference: A predictor of testosterone concentration and certain attributes of seminal vesicles influencing buffalo male fertility. Vet. World, 11(6): 739-747. DOI: 10.14202/vetworld.2018.739-747

Martins, C.F., R. Rumpf, D.C. Pereira and M.N. Dode. 2007. Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in vitro embryo production. Animal. Reprod. Sci., 101(3-4): 326-331. DOI: 10.1016/j.anireprosci.2007.01.018

Nichi, M., T. Rijsselare, A. Van Soom, J.B.P. De Clercq, I.G.F. Goovaerts, V.H. Barnabe and P.E.J. Bols. 2007. Effect of bull epididymis storage conditions on cryopreserved epididymal sperm in vitro fertility and lipid peroxidation status. The 33rd Annual International Embryo Transfer Society, Kyoto, Japan.

Oennes, H., F.A. Bouzebda, Z. Bouzebda, S. Medjedoub, A. Djaout and M.A. Smadi. 2019. Effect of testicle postmortem storage on goat epididymal sperm quality: The first step towards cryobank for local Algeria breeds. Reveu. Med. Vet., 170(7-9): 184-192. Available on: https://www.researchgate.net/profile/Ouennes-Houria/publication/336232875_RMV170_184_192/links/5d95dd66458515c1d38ffe26/RMV170-184-192.pdf

Pant, H.C., R.K. Sharma, S.H. Patel, H.R. Shukla, A.K. Mittal, R. Kasiraj, A.K. Misra and J.H. Prabhakar. 2003. Testicular development and its relationship to semen production in Murrah buffalo bulls. Theriogenology, 60(1): 27-34. DOI: 10.1016/s0093-691x(02)01037-3

Ranga, L.C., R.K. Chandolia, L. Singh and R. Dutt. 2020. Testicular ultrasonogram pixel intensity in developing buffalo calves/bulls. Veterinary Research International, 8(4): 302-307. Available on: https://www.researchgate.net/profile/Ravi-Dutt-3/publication/348326498_Testicular_Ultrasonogram_Pixel_Intensity_in_Developing_Buffalo_CalvesBulls/links/5ff82806a6fdccdcb83b75b9

/Testicular-Ultrasonogram-Pixel-Intensity-in-Developing-Buffalo-Calves-Bulls.pdf

Saurabh, S.S., A. Kumar, S. Verma, P. Sharma and V. Gautam. 2018. Study of testicular biometry and its correlation with seminal attributes in Murrah bull. Pharma Innovation Journal, 7(6): 251-256.

Siqueira, J.B., E. Oba, R.O. Pinho, E.F. Castilho, R.F. Bittencourt, S.S. Assaf and J.D. Guimaraes. 2007. Gonadic sperm reserve in buffaloes created under tropical condition. Ital. J. Anim. Sci., 6(2S): 615-618. DOI: 10.4081/ijas.2007.s2.615

Tomar, N.S. 1997. Artificial Insemination and Reproduction in Cattle and Buffalo, 4rd ed. Saroj Prakashan, Allahabad, India.

Valete, E.J.P., E.R.S. Maylem, M.B. Ocampo, E.A. Abella and L.C. Ocampo. 2015. Post mortem viability of epididymal sperm from Philippine native water buffalo (Bubalus bubalis). Journal of Agricultural Technology, 11(8): 2283-2294. Available on: http://ijat-aatsea.com/pdf/v11_n8_15_DecemberSpecialissue/073-%20Edeneil%20Jerome%20P.%20Valete%20-S5.pdf

Varghese, M.R., M.A. Kataktalware, S. Jeyakumar, D.N. Das, K.P. Ramesha, and P.R. Wankhade. 2019. Testicular biometry and its relationship with age and body weight in young Deoni males. Indian J. Anim. Res., 53(12): 1624-1628. DOI: 10.18805/ijar.B-3703

Yadav, S.K., P. Singh, P. Kumar, S.V. Singh, A. Singh and S. Kumar. 2019. Scrotal infrared thermography and testicular biometry: Indicator of semen quality in Murrah buffalo bulls. Anim. Reprod. Sci., 209: 106145. DOI: 10.1016/J.ANIREPROSCI.2019.106145

Younis, M., H.A. Samad, N. Ahmad and I. Ahmad. 2003. Effects of age and season on the body weight, scrotal circumference and libido of Nili-Ravi buffalo bulls maintained at the semen production unit, Qadirabad. Pak. Vet. J., 23(2): 59-65.

Yulnawati, H. Maheshwari, Herdis and M. Rizal. 2009. Viability and plasma membrane integrity of the spotted buffalo epididymal spermatozoa after thawing with the addition of dextrose into the extender. Biotropia, 16(1): 21-27. DOI: 10.11598/btb.2009.16.1.63

Downloads

Published

2023-12-31

How to Cite

Ashotosh, K., Srivastava, S., Kumar, R., & Kumar, P. (2023). Testicular biometry and certain aspects of epididymal semen of abattoir derived Murrah bull testes. Buffalo Bulletin, 42(4), 555–562. https://doi.org/10.56825/bufbu.2023.4244312

Issue

Section

Original Article

Most read articles by the same author(s)

1 2 > >>