The response of blood plasma metabolite concentrations of anestrus lactating buffalo to CIDR-eCG with or without melatonin treatment

Authors

  • Tamer Awad Ramadan Animal Production Research Institute, Agricultural Research Center, Giza, Egypt
  • Rakesh Kumar Sharma    Animal Physiology and Reproduction Division, Indian Council of Agricultural Research, Central Institute for Research on Buffaloes, Haryana, India
  • Sushil Kumar Phulia Animal Physiology and Reproduction Division, Indian Council of Agricultural Research, Central Institute for Research on Buffaloes, Haryana, India
  • Ashok Kumar Balhara Animal Physiology and Reproduction Division, Indian Council of Agricultural Research, Central Institute for Research on Buffaloes, Haryana, India
  • Sarvpreet Singh Ghuman Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India
  • Inderjeet Singh Animal Physiology and Reproduction Division, Indian Council of Agricultural Research, Central Institute for Research on Buffaloes, Haryana, India

DOI:

https://doi.org/10.56825/bufbu.2022.4124518

Keywords:

Bubalus bubalis, Buffaloes, Melatonin, CIDR-eCG protocol, Blood metabolites, Lactating buffalo

Abstract

The objective of this study was to compare the efficiency of CIDR-eCG protocol with or without melatonin treatment on blood plasma metabolites in anestrus Murrah buffaloes during out-of-breeding season. Twelve lactating Murrah buffaloes were allotted to control and treated groups each of six animals. Treated buffaloes were implanted with melatonin (18 mg melatonin / 50 kg body weight) for 45 days, then animals of both groups received CIDR for 9 days. All animals received 500 IU eCG intramuscularly one day before CIDR removal and 10 µg GnRH on the day of AI. The animals were subjected to estrus detection daily as per farm routine. At weekly intervals, blood samples were collected to determine the concentration of blood plasma metabolites over 84 days’ period between June and September. All buffaloes exhibited estrus after CIDR removal. CIDR-eCG preceded with melatonin treatment increased plasma total protein, albumin, cholesterol and HDL-cholesterol (P<0.05) in comparison to only CIDR treated control. Buffaloes treated with melatonin plus CIDR exhibited increased (P<0.01) plasma glucose on days 28, 53 and 55 and reduction (P<0.01) in HDL and AST at time of pregnancy diagnosis, while plasma concentration of phosphorus was the highest (P<0.05) at day 84. In conclusion, CIDR-eCG treatment preceded with melatonin implantation improved reproductive performance and altered blood metabolite composition of lactating buffaloes during out-of-breeding season as compared to CIDR-eCG only.

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Author Biographies

Tamer Awad Ramadan, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt

Tamer Awad Ramadan

tamereweda1174@yahoo.com

Rakesh Kumar Sharma   , Animal Physiology and Reproduction Division, Indian Council of Agricultural Research, Central Institute for Research on Buffaloes, Haryana, India

 Rakesh Kumar Sharma

 

Sushil Kumar Phulia, Animal Physiology and Reproduction Division, Indian Council of Agricultural Research, Central Institute for Research on Buffaloes, Haryana, India

Sushil Kumar Phulia

Ashok Kumar Balhara, Animal Physiology and Reproduction Division, Indian Council of Agricultural Research, Central Institute for Research on Buffaloes, Haryana, India

Ashok Kumar Balhara

Sarvpreet Singh Ghuman, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India

Sarvpreet Singh Ghuman
Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India

Inderjeet Singh, Animal Physiology and Reproduction Division, Indian Council of Agricultural Research, Central Institute for Research on Buffaloes, Haryana, India

Inderjeet Singh

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Published

2022-06-26

How to Cite

Ramadan, T. A., Sharma  ,R.K., Phulia, S. K., Balhara, A. K., Ghuman, S. S., & Singh, I. (2022). The response of blood plasma metabolite concentrations of anestrus lactating buffalo to CIDR-eCG with or without melatonin treatment. Buffalo Bulletin, 41(2), 351–362. https://doi.org/10.56825/bufbu.2022.4124518

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