Effect of melatonin implantation prior to ovsynch plus cidr protocol on subsequent ovarian activity and pregnancy rate in summer anestrous Murrah buffaloes


  • Sarvpreet Singh Ghuman College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
  • Mrigank Honparkhe Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
  • Bilawal Singh Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India




Bubalus bubalis, buffaloes, melatonin, ovarian activity, pregnancy, summer


For assessing the impact of melatonin pre-treatment in summer anestrous Murrah buffaloes on subsequent reproductive activity of a fixed time artificial insemination (FTAI) protocol, 83 anestrous Murrah buffaloes were subcutaneously inserted 2x4 mm size absorbable melatonin (MT) implants (18 mg/50 kg b wt) at the base of left ear and 38 Murrah buffaloes were used as non-implanted controls. On day 35 post-MT, the Treatment and Control group Murrah buffaloes were subjected to FTAI protocol (Ovsynch plus CIDR). Ovarian ultrasonography and jugular vein blood sampling was carried out on day 7 and 10 of protocol and on day 7-post AI. Overall pregnancy rate on day 90 post-AI in MT group was higher compared to Control group (55.4 vs 31.6%, P<0.05), along with the higher (P>0.05) plasma progesterone on day 7 of FTAI protocol in Murrah buffaloes of the former group which conceived subsequently compared to their non-pregnant counterparts. Moreover, MT group had higher (P>0.05) increase in dominant follicle diameter between day 7 and 10 of protocol compared to Control group. On day 7 post-AI, the luteal profile in pregnant Murrah buffaloes of MT group was better (P<0.05) compared to their counterparts in Control group (CL: 14.80±0.70 vs 10.82±0.46 mm, P4: 1.54±0.07 vs 1.04±0.08 ng/ml). In conclusion, melatonin pre-treatment in summer anestrous Murrah buffaloes could be associated with better luteal activity and higher pregnancy rate in the Ovsynch+CIDR protocol.


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How to Cite

Ghuman, S. S., Honparkhe, M., & Singh, B. (2023). Effect of melatonin implantation prior to ovsynch plus cidr protocol on subsequent ovarian activity and pregnancy rate in summer anestrous Murrah buffaloes. Buffalo Bulletin, 42(2), 263–269. https://doi.org/10.56825/bufbu.2023.4223586



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