Assessment of oxidative stress parameters in summer anestrous buffaloes exhibiting differential fertility following melatonin implants treatment

Sarvpreet Singh Ghuman; Ranjana Singh Cheema; Mrigank Honparkhe

doi:10.56825/bufbu.2024.4313563

Authors

Sarvpreet Singh Ghuman Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India
Ranjana Singh Cheema Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India
Mrigank Honparkhe Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India

DOI:

https://doi.org/10.56825/bufbu.2024.4313563

Keywords:

Bubalus bubalis, buffaloes, anestrus, antioxidant, fertility, melatonin

Abstract

For evaluating the impact of melatonin implants treatment during non-breeding season to ameliorate oxidative stress, 132 anestrous buffaloes were subcutaneously inserted with 2x4 mm absorbable slow-release melatonin implants (18 mg/50 kg b wt) at the base of left ear and 60 buffaloes were used as control. Ovarian ultrasonography and jugular vein blood sampling were carried out at 7-day interval till day 35 post-treatment or till ovulation, whichever was earlier. Control and implanted buffaloes were subjected to artificial insemination (AI) at overt or induced estrus followed by pregnancy diagnosis at day 90 post-AI. Erythrocytic lipid peroxidation (LPO) values were reduced (P<0.05) in implanted buffaloes from day 21 post-treatment onwards when compared to their pre-treatment and Control group values. However, the concentrations of erythrocytic glutathione peroxidase (GPx), glutathione reductase and superoxide dismutase (SOD) were invariably higher (P<0.05) following treatment as compared to their pre-treatment and Control group values. The buffaloes ovulating in Control or Treatment group revealed higher (P<0.05) erythrocytic GPx in the Latter group. Also, between pregnant counterparts of Control and Treatment group, the Latter group buffaloes exhibited low (P<0.05) erythrocytic LPO, and high (P<0.05) erythrocytic GPx, SOD and catalase. It can be concluded that melatonin implants treatment was successful for mitigating the oxidative stress in summer anestrous buffaloes, and the status of oxidative stress parameters following treatment was better in buffaloes that ovulated or conceived subsequently.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Asadpour, R., R. Jafari and H. Tayefi-Nasrabadi. 2011. Effect of various levels of catalase antioxidant in semen extenders on lipid peroxidation and semen quality after the freeze-thawing bull semen. Vet. Res. Forum, 2(4): 218-221.

Boni, R., L. Santella, B. Dale, S. Roviello, R. Di Palo and V. Barbieri. 1992. Maturazione in vitro di oociti buffalini: Indagine ultrastrutturale. Acta Med. Vet., 38: 153-161.

D’Occhio, M.J., S.S. Ghuman, G. Neglia, G. Valle, P.S. Baruselli, L. Zicarelli, J.A. Visintin, M. Sarkar and G. Campanile. 2020. Exogenous and endogenous factors in seasonality of reproduction in buffalo: A review. Theriogenology, 150: 186-192. DOI: 10.1016/j.theriogenology.2020.01.044

El-Battawy, K.A. and M. Sckalcki. 2015. Seminal Profile, antioxidant enzymes activities and levels of testosterone in Tyrolean mountain rams after melatonin implantation. Middle East Journal of Applied, 5(4): 1232-1238. Available on: https://www.curresweb.com/mejas/mejas/2015/1232-1238.pdf

Forcada, F., J.A. Abecia, O. Zuniga and J.M. Lozano. 2002. Variation in the ability of melatonin implants inserted at two different times after the winter solstice to restore reproductive activity in reduced seasonality ewes. Aust. J. Agri. Res., 53(2): 167-173. DOI: 10.1071/AR00172

Gavella, M., V. Lipovac, M. Vucic and B. Rocic. 1996. Relationship of sperm superoxide dismutase-like activity with other sperm-specific enzymes and experimentally induced lipid peroxidation in infertile men. Andrologia, 28(4): 223-229. DOI: 10.1111/j.1439-0272.1996.tb02787.x

Ghuman, S.P.S., J. Singh, M. Honparkhe, D. Dadarwal, G.S. Dhaliwal and A.K. Jain. 2010. Induction of ovulation of ovulatory size non-ovulatory follicles and initiation of ovarian cyclicity in summer anoestrous buffalo heifers (Bubalus bubalis) using melatonin implants. Reprod. Domest. Anim., 45(4): 600-607. DOI: 10.1111/j.1439-0531.2008.01310.x

Guérin, P., M.S. El and Y. Ménézo. 2001. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Human Reprod. Update, 7(2): 175. DOI: 10.1093/humupd/7.2.175

Jan, M.H., G.K. Das, F.A. Khan, J. Singh, S.T. Bashir, S. Khan, J.K. Prasad, S. Mehrotra, M.C. Pathak, G. Singh and M. Sarkar. 2014. Evaluation of follicular oxidant-antioxidant balance and oxidative damage during reproductive acyclicity in water buffalo (Bubalus bubalis). Asian Pacific Journal of Reproduction, 3(1): 35-40. DOI: 10.1016/S2305-0500(13)60182-7

Kumar, A., S. Mehrotra, G. Singh, K. Narayanan, G.K. Das, Y.K. Soni, M. Singh, A.S. Mahla, N. Srivastava and M.R. Verma. 2015. Sustained delivery of exogenous melatonin influences biomarkers of oxidative stress and total antioxidant capacity in summer-stressed anestrous water buffalo (Bubalus bubalis). Theriogenology, 83: 1402-1407. DOI: 10.1016/j.theriogenology.2014.12.023

Laloraya, M., P.G. Kumar and M.M. Laloraya. 1988. Changes in the levels of superoxide anion radical and superoxide dismutase during the estrous cycle of Rattus norvegicus and induction of superoxide dismutase in rat ovary by lutropin. Biochem. Bioph. Res. Co., 157(1): 1146-1153. DOI: 10.1016/s0006-291x(88)80025-1

Lochan, S., M. Honparkhe, R.S. Cheema, A. Kumar, S.P.S. Ghuman and P.S. Brar. 2020. Ameliorating postpartum reproductive cyclicity using Exogenous melatonin implant in water buffalo (Bubalus bubalis). Indian J. Anim. Sci., 90(2), 181-184. DOI: 10.56093/ijans.v90i2.98772

Medrano, A., C.F. Contreras, F. Herrera and A. Alcantar-Rodriguez. 2017. Melatonin as an antioxidant preserving sperm from domestic animals. Asian Pacific Journal of Reproduction, 6(6): 241-246. DOI: 10.4103/2305-0500.217317

Nagina, G., A. Asima, U. Nemat and A. Shamim. 2016. Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes. Open Veterinary Journal, 6(2): 128-134. DOI: 10.4314/ovj.v6i2.9

Pandey, A.K., P. Gunwant, N. Soni, Kavita, S. Kumar, A. Kumar, A. Magotra, I. Singh, J.B. Phogat, R.K. Sharma, Y. Bangar, S.P.S. Ghuman and S.S. Sahu. 2019. Genotype of MTNR1A gene regulates the conception rate following melatonin treatment in water buffalo. Theriogenology, 128: 1-7. DOI: 10.1016/j.theriogenology.2019.01.018

Ramadan, T.A., R.K. Sharma, S.K. Phulia, A.K. Balhara, S.S. Ghuman and I. Singh. 2016. Manipulation of reproductive performance of lactating buffaloes using melatonin and controlled internal drug release device treatment during out-of-breeding season under tropical conditions. Theriogenology, 86(4): 1048-1053. DOI: 10.1016/j.theriogenology.2016.03.034

Rapoport, R., D. Sklan, D. Wolfenson, A. Shaham-Albalancy and I. Hanukoglu. 1998. Antioxidant capacity is correlated with steroidogenic status of the corpus luteum during the bovine estrous cycle. Biochem. Biophys. Acta, 1380(1): 133-140. DOI: 10.1016/S0304-4165(97)00136-0

Reina, M. and A. Martinez. 2018. A new free radical scavenging cascade involving melatonin and three of its metabolites (3OHM, AFMK and AMK). Comput. Theor. Chem., 1123: 111-118. DOI: 10.1016/j.comptc.2017.11.017

Reiter, R.J. 1993. The melatonin rhythm: both a clock and a calendar. Experientia, 49(8): 654-664. DOI: 10.1007/BF01923947

Reiter, R.J. 1998. Melatonin and human reproduction. Ann. Med., 30(1): 103-108. DOI: 10.3109/07853899808999391

Rock, C.L., R.A. Jacob and P.E. Bowen. 1996. Update on the biological characteristics of the antioxidant micronutrients: vitamin C, vitamin E, and the carotenoids. J. Am. Diet. Assoc., 96(7): 693-702. DOI: 10.1016/S0002-8223(96)00190-3

Rodriguez, C., J.C. Mayo, R.M. Sainz, I. Antolin, F. Herrera, V. Martin and R.J. Reiter. 2004. Regulation of antioxidant enzymes: A significant role for melatonin. J. Pineal Res., 36(1): 1-9. DOI: 10.1046/j.1600-079x.2003.00092.x

Singh, B., S.P.S. Ghuman, R.S. Cheema and A.K. Bansal. 2016. Melatonin implant induces estrus and alleviates oxidative stress in summer anestrus buffalo. Indian J. Anim. Reprod., 37(2): 28-32.

Van Zoeren-Grobben, D., J.H.N. Lindeman, E. Houdkamp, R. Bland, J. Schrijver and H.M. Berger. 1994. Postnatal changes in plasma chain-breaking antioxidants in healthy preterm infants fed formula and/or human milk. Am. J. Clin. Nutr., 60(6): 900-906. DOI: 10.1093/ajcn/60.6.900