Diagnosis of the Mycobacterium avium subspecies paratuberculosis in tissues of slaughtered buffaloes using fluorescent antibody test

Authors

  • Gaya Prasad Jatav Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Madhya Pradesh, India
  • Shoor Vir Singh Department of Biotechnology, GLA University, Uttar Pradesh, India
  • Kundan Kumar Chaubey Department of Biotechnology, GLA University, Uttar Pradesh, India
  • Saurabh Gupta Department of Biotechnology, GLA University, Uttar Pradesh, India
  • Anantrao Kashiram Jayraw Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Madhya Pradesh, India
  • Vivek Agrawal Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Madhya Pradesh, India
  • Mukesh Shakya Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Madhya Pradesh, India
  • Rashmi Choudhary Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Madhya Pradesh, India
  • Nirmala Jamra Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Madhya Pradesh, India

DOI:

https://doi.org/10.56825/bufbu.2024.4344617

Keywords:

Bubalus bubalis, buffaloes, fluorescent antibody test, MAP, Malwa region

Abstract

Paratuberculosis (pTB) caused by Mycobacterium avium subsp. paratuberculosis (MAP), infects domestic livestock leading to chronic diarrhoea (persistent, intermittent or no diarrhoea) and loss in body condition and weights. It is a major disease that adversely affects health and productivity resulting in colossal monetary losses to the livestock industry throughout the world. Association of MAP with inflammatory bowel disease / Crohn’s disease (CD) in humans, established its zoonotic potential and public health significance. Tissues (corrugated mucosal folds) of 20 buffaloes (both the sexes, aged 1 to 10 years) from Malwa division of Madhya Pradesh were anlyzed by fluorescent antibody test (FAT).  Out of 20 tissue smears screened, 11 (55.0%) were positive for MAP by FAT.

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References

D’Haese, E., I. Dumon, H. Werbrouck, V. D’Jonghe and L. Herman. 2005. Improved detection of mycobacterium paratuberculosis in milk. J. Dairy Res., 72(S1): 125-128. DOI: 10.1017/s0022029905001226

Gilmour, N.J. and K.W. Angus. 1976. The specificity and sensitivity of the fluorescent antibody test in cattle experimentally infected with Mycobacterium avium and Mycobacterium johnei. Res. Vet. Sci., 20(1): 6-9. DOI: 10.1016/S0034-5288(18)33470-2

Goudswaard, J., R.H. Van Dam and A.P.M.G. Bertens. 1977. Comparison of fluorescent antibody tests for tuberculosis and paratuberculosis with antigens coupled to insoluble spheres or taken up by macrophages. Vet. Microbiol., 2(1): 39-47. DOI: 10.1016/0378-1135(77)90030-X

Jatav, G.P., U.K. Garg, S. Shukla, D. Chhabra, A.K. Jayraw, N. Shrivastava, S.K. Karmore and V. Agrawal. 2017. Incidence of Mycobacterium avium subsp. paratuberculosis (MAP) infection in slaughtered buffaloes (Bubalus bubalis) of Malwa region of Madhya Pradesh. Indian Journal of Veterinary Sciences and Biotechnology, 13(2): 16-20.

Jatav, G.P., A.K. Jayraw, N. Shrivastava, R. Sikrodia, V. Agrawal and S.K. Karmore. 2018a. Detection of Mycobacterium avium subsp. Paratuberculosis (MAP) in faeces of buffaloes (Bubalus bubalis) from Malwa region of Madhya Pradesh. International Journal of Livestock Research, 8: 73-78. DOI: 10.5455/ijlr.20170830105736

Jatav, G.P., S. Shukla, A.K. Jayraw, D. Chhabra, V. Agrawal, S.V. Singh and S.K. Karmore. 2018b. Detection of anti-MAP antibodies in serum samples of slaughtered buffaloes (Bubalus bubalis) by indigenous ELISA kit in Malwa region of Madhya Pradesh. J. Anim. Res., 8(2): 1-3. Available on: https://ndpublisher.in/admin/issues/JARv8n2x.pdf

McNab, W.B., A.H. Meek, S.W. Martin and J.R. Duncan. 1991. Associations between dairy production indices and lipoarabinomannan enzyme-immunoassay results for paratuberculosis. Can. J. Vet. Res., 55(4): 356-361.

Singh, S.V., B.J. Stephen, M. Singh, S. Gupta, K.K. Chaubey, Sahzad, S. Jayaraman, T.K. Sachan, G.K. Aseri, M. Dutta, J.S. Sohal, K. Dhama, S.Y. Mukartal and D. Rathnamma. 2016. Evalution of “indirect fluorescent antibody test” as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock. Journal of Experimental Biology and Agricultural Sciences, 4(5): 533-540. Available on: https://www.cabidigitallibrary.org/doi/pdf/10.5555/20163325717

Stephen, B.J., M. Singh, S.V. Singh, S. Gupta, K.K. Choubey, Sahzad, S. Jayaraman, M. Jain, J.S. Sohal, S.Y. Mukartal and K. Dhama. 2016. Bio-contamination estimation of MAP in fresh cottage cheese (Paneer) sold in rural, semi urban and peri urban regions of south U.P. using multiple diagnostic tests. Advances in Animals and Veterinary Sciences, 4(8): 441-449. DOI: 10.14737/journal.aavs/2016/4.8.441.448

Sweeney, R.W. 1996. Transmission of paratuberculosis. Vet. Clin. N. Am. Food-A., 12(2): 305-312. DOI: 10.1016/s0749-0720(15)30408-4

fluorescent green colour rods of MAP organisms (FAT, 1000X) (arrows)

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Published

2024-12-31

How to Cite

Jatav, G. P., Singh, S. V., Chaubey, K. K., Gupta, S., Jayraw, A. K., Agrawal, V., … Jamra, N. (2024). Diagnosis of the Mycobacterium avium subspecies paratuberculosis in tissues of slaughtered buffaloes using fluorescent antibody test. Buffalo Bulletin, 43(4), 529–533. https://doi.org/10.56825/bufbu.2024.4344617

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