Effect of cholesterol loaded cyclodextrin on cryosurvivability of buffalo spermatozoa
Keywords:
Bubalus bubalis, Cholesterol, buffaloes, Cyclodextrin, cholesterol, Concentration, cyclodextrin, Cryosurvival, concentration, Buffalo, cryosurvival, Spermatozoa, spermatozoaAbstract
The study was carried out to understand the effect of various concentrations of cholesterol loaded cyclodextrin (CLC) on cryosurvivability of buffalo spermatozoa. Twenty four ejaculates with mass motility ≥ 3+ from 4 bulls (6 from each bull) were collected as per standard procedure. Each ejaculate was split into four groups viz., Group I (control: extended with Tris-egg yolk-glycerol extender upto 80×106 sperm/ml), Group II (treated with CLC 1 mg/ml), Group III (treated with CLC 2 mg/ml) and Group IV (treated with CLC 3 mg/ml). Semen samples of Group II, III or IV were incubated with CLC at 37oC for 15 minutes for the entry of cholesterol into sperms and finally diluted upto 80×106 sperm/ml. The results revealed that post-dilution and after freezing at 0.7 and 30 days of cryopreservation motility, live and dead, acrosomal integrity and hypo-osmotic swelling test were significantly (P<0.05) higher in Group III as compared to all other groups. However, significantly (P<0.05) higher percentage of abnormal sperm was observed in control group as compared to other groups. Therefore, addition of CLC 2 mg/ml in extender during buffalo semen cryopreservation has beneficial role in improvement of semen quality.
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References
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Campbell, R.G., J.L. Hancock and L. Rothschild. 1953. Counting live and dead bull spermatozoa. J. Exp. Biol., 30: 44-45. Available on: https://jeb.biologists.org/content/jexbio/30/1/44.full.pdf
Cerolini, S., A. Maldjian, F. Pizzi and T.M. Gliozzi. 2001. Changes in sperm quality and lipid composition during cryopreservation of boar semen. Reproduction, 121(3): 395-401.
Chakrabarty, J., D. Banerjee, D. Pal, J. De, A. Ghosh and G.C. Majumder. 2007. Shedding off specific lipid constituents from sperm cell membrane during cryopreservation. Cryobiology, 54(1): 27-35. DOI: 10.1016/j.cryobiol.2006.10.191
Darin-Bennett, A. and I.G. White. 1977. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology, 14(4): 466-470. DOI: 10.1016/0011-2240(77)90008-6
Glazar, A.I., S.F. Mullen, J. Liu, J.D. Benson, J.K. Critser, E.L. Squires and J.K. Graham. 2009. Osmotic tolerance limits and membrane permeability characteristics of stallion spermatozoa treated with cholesterol. Cryobiology, 59(2): 201-20. DOI: 10.1016/j.cryobiol.2009.07.009
Jeyendran, R.S., Vander, H.H. Ven., M. Parez-pelaez, B.G. Crabo and L.J.D. Zaneweld. 1984. Development of an assay to assess the functional integrity of the human membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70(1): 219-228. DOI: 10.1530/jrf.0.0700219
Klein, V., G. Gimpl and F. Fahrenholz. 1995. Alteration of the myometrial plasma membrane cholesterol content with cyclodextrin modulates the binding affinity of the oxytocin receptor. Biochemistry, 34(42): 13784-13793. DOI: 10.1021/bi00042a009
Lone, S.A., J.K. Prasad, S.K. Ghosh, G.K. Das, B. Balamurugan, N. Kumar, R. Katiyar and M.R. Verma. 2016a. Effect of different post-thaw incubation periods on motility and viability of cholesterol loaded cyclodextrin treated buffalo semen. Indian J. Dairy Sci., 69(4): 479-482.
Lone, S.A., J.K. Prasad, S.K. Ghosh, N. Kumar, S.A. Bhat and G.K. Das. 2016b. Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen. Indian J. Anim. Sci., 86(11): 1255-1258.
Lone, S.A., J.K. Prasad, S.K. Ghosh, G.K. Das, N. Kumar, B. Balamurugan, R. Katiyar and M.R. Verma. 2016c. Effect of cholesterol loaded cyclodextrin (CLC) on lipid peroxidation and reactive oxygen species levels during cryopreservation of buffalo (Bubalus bubalis) spermatozoa. Asian Pacific Journal of Reproduction, 5(6): 476-480. DOI: 10.1016/j.apjr.2016.10.003
Longobard, V., Z. Gianluig, A. Salzano, De C. Canditiis, A. Cammarano, De L. Luise, M.V. Puzio, G. Neglia and B. Gasparrini. 2017. Resveratrol prevents capacitation-like changes and improves in vitro fertilizing capability of buffalo frozen-thawed sperm. Theriogenology, 88: 1-8. DOI: 10.1016/j.theriogenology.2016.09.046
Moore, A.I., L. Edward, J. Squires and K. Graham. 2005. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology, 51(3): 241-249. DOI: 10.1016/j.cryobiol.2005.07.004
Purdy, P.H. and J.K. Graham. 2004. Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology, 48(1): 36-45. DOI: 10.1016/j.cryobiol.2003.12.001
Rajoriya, J.S., J.K. Prasad, S.K. Ghosh, S. Ramteke, N.C. Barik, G.K. Das and M. Pande. 2014. Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio. Vet. World, 7(9): 702-706. DOI: 10.14202/vetworld.2014.702-706
Rajoriya, J.S., J.K. Prasad, S.S. Ramteke, P. Perumal, S.K. Ghosh, M. Singh, M. Pande and N. Srivastava. 2016. Enriching membrane cholesterol improves stability and cryosurvival of buffalo spermatozoa. Anim. Reprod. Sci., 164: 72-81. DOI: 10.1016/j.anireprosci.2015.11.014
S.A.S, 2011. SAS 9.3 System Options: Reference, 2nd ed. Statistical Analysis software Institute Inc. Cary, North Carolina, USA.
Serin, I., M. Aksoy and A. Ceylan. 2011. Cholesterol-loaded cyclodextrin inhibits premature acrosomal reactions in liquid-stored spermatozoa. Anim. Reprod. Sci., 123(1-2): 106-111. DOI: 10.1016/j.anireprosci.2010.10.014
Srivastava, N., S.K. Srivastava, S.K. Ghosh, L.P. Singh, J.K. Prasad, A. Kumar, P. Perumal, A. Jerome and A. Thamizharasane. 2012. Sequestration of PDC-109 protein improves freezability of crossbred bull spermatozoa. Anim. Reprod. Sci., 131(1-2): 54-62. DOI: 10.1016/j.anireprosci.2012.02.003
Therien, I., G. Bleau and P. Manjunath. 1995. Phosphatidylcholine binding proteins of bovine seminal plasma modulate capacitation of spermatozoa by heparin. Biol. Reprod., 52(6): 1372-1379. DOI: 10.1095/biolreprod52.6.1372
Watson, P.F. 1975. Use of Giemsa stain to detect changes in the acrosome of frozen ram spermatozoa. Vet. Rec., 97(1): 12-15. DOI: 10.1136/vr.97.1.12
White, I.G. 1993. Lipids and calcium uptake of sperm in relation to cold shock and preservation: A review. Reprod. Fert. Develop., 5(6): 639-658. DOI: 10.1071/rd9930639
Bailey, J.L., C. Lessard, J. Jacques, C. Breque, I. Dobrinski, W. Zeng and H.L. Galantino-Homer. 2008. Cryopreservation of boar semen and its future importance to the industry. Theriogenology, 70(8): 1251-1259. DOI: 10.1016/j.theriogenology.2008.06.014
Campbell, R.G., J.L. Hancock and L. Rothschild. 1953. Counting live and dead bull spermatozoa. J. Exp. Biol., 30: 44-45. Available on: https://jeb.biologists.org/content/jexbio/30/1/44.full.pdf
Cerolini, S., A. Maldjian, F. Pizzi and T.M. Gliozzi. 2001. Changes in sperm quality and lipid composition during cryopreservation of boar semen. Reproduction, 121(3): 395-401.
Chakrabarty, J., D. Banerjee, D. Pal, J. De, A. Ghosh and G.C. Majumder. 2007. Shedding off specific lipid constituents from sperm cell membrane during cryopreservation. Cryobiology, 54(1): 27-35. DOI: 10.1016/j.cryobiol.2006.10.191
Darin-Bennett, A. and I.G. White. 1977. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology, 14(4): 466-470. DOI: 10.1016/0011-2240(77)90008-6
Glazar, A.I., S.F. Mullen, J. Liu, J.D. Benson, J.K. Critser, E.L. Squires and J.K. Graham. 2009. Osmotic tolerance limits and membrane permeability characteristics of stallion spermatozoa treated with cholesterol. Cryobiology, 59(2): 201-20. DOI: 10.1016/j.cryobiol.2009.07.009
Jeyendran, R.S., Vander, H.H. Ven., M. Parez-pelaez, B.G. Crabo and L.J.D. Zaneweld. 1984. Development of an assay to assess the functional integrity of the human membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70(1): 219-228. DOI: 10.1530/jrf.0.0700219
Klein, V., G. Gimpl and F. Fahrenholz. 1995. Alteration of the myometrial plasma membrane cholesterol content with cyclodextrin modulates the binding affinity of the oxytocin receptor. Biochemistry, 34(42): 13784-13793. DOI: 10.1021/bi00042a009
Lone, S.A., J.K. Prasad, S.K. Ghosh, G.K. Das, B. Balamurugan, N. Kumar, R. Katiyar and M.R. Verma. 2016a. Effect of different post-thaw incubation periods on motility and viability of cholesterol loaded cyclodextrin treated buffalo semen. Indian J. Dairy Sci., 69(4): 479-482.
Lone, S.A., J.K. Prasad, S.K. Ghosh, N. Kumar, S.A. Bhat and G.K. Das. 2016b. Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen. Indian J. Anim. Sci., 86(11): 1255-1258.
Lone, S.A., J.K. Prasad, S.K. Ghosh, G.K. Das, N. Kumar, B. Balamurugan, R. Katiyar and M.R. Verma. 2016c. Effect of cholesterol loaded cyclodextrin (CLC) on lipid peroxidation and reactive oxygen species levels during cryopreservation of buffalo (Bubalus bubalis) spermatozoa. Asian Pacific Journal of Reproduction, 5(6): 476-480. DOI: 10.1016/j.apjr.2016.10.003
Longobard, V., Z. Gianluig, A. Salzano, De C. Canditiis, A. Cammarano, De L. Luise, M.V. Puzio, G. Neglia and B. Gasparrini. 2017. Resveratrol prevents capacitation-like changes and improves in vitro fertilizing capability of buffalo frozen-thawed sperm. Theriogenology, 88: 1-8. DOI: 10.1016/j.theriogenology.2016.09.046
Moore, A.I., L. Edward, J. Squires and K. Graham. 2005. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology, 51(3): 241-249. DOI: 10.1016/j.cryobiol.2005.07.004
Purdy, P.H. and J.K. Graham. 2004. Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology, 48(1): 36-45. DOI: 10.1016/j.cryobiol.2003.12.001
Rajoriya, J.S., J.K. Prasad, S.K. Ghosh, S. Ramteke, N.C. Barik, G.K. Das and M. Pande. 2014. Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio. Vet. World, 7(9): 702-706. DOI: 10.14202/vetworld.2014.702-706
Rajoriya, J.S., J.K. Prasad, S.S. Ramteke, P. Perumal, S.K. Ghosh, M. Singh, M. Pande and N. Srivastava. 2016. Enriching membrane cholesterol improves stability and cryosurvival of buffalo spermatozoa. Anim. Reprod. Sci., 164: 72-81. DOI: 10.1016/j.anireprosci.2015.11.014
S.A.S, 2011. SAS 9.3 System Options: Reference, 2nd ed. Statistical Analysis software Institute Inc. Cary, North Carolina, USA.
Serin, I., M. Aksoy and A. Ceylan. 2011. Cholesterol-loaded cyclodextrin inhibits premature acrosomal reactions in liquid-stored spermatozoa. Anim. Reprod. Sci., 123(1-2): 106-111. DOI: 10.1016/j.anireprosci.2010.10.014
Srivastava, N., S.K. Srivastava, S.K. Ghosh, L.P. Singh, J.K. Prasad, A. Kumar, P. Perumal, A. Jerome and A. Thamizharasane. 2012. Sequestration of PDC-109 protein improves freezability of crossbred bull spermatozoa. Anim. Reprod. Sci., 131(1-2): 54-62. DOI: 10.1016/j.anireprosci.2012.02.003
Therien, I., G. Bleau and P. Manjunath. 1995. Phosphatidylcholine binding proteins of bovine seminal plasma modulate capacitation of spermatozoa by heparin. Biol. Reprod., 52(6): 1372-1379. DOI: 10.1095/biolreprod52.6.1372
Watson, P.F. 1975. Use of Giemsa stain to detect changes in the acrosome of frozen ram spermatozoa. Vet. Rec., 97(1): 12-15. DOI: 10.1136/vr.97.1.12
White, I.G. 1993. Lipids and calcium uptake of sperm in relation to cold shock and preservation: A review. Reprod. Fert. Develop., 5(6): 639-658. DOI: 10.1071/rd9930639
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Published
2021-03-26
How to Cite
Bishist, R., Raina, V. S., Bhakat, M., Lone, S. A., Mohanty, T. K., Sinha, R., & Kumar, R. (2021). Effect of cholesterol loaded cyclodextrin on cryosurvivability of buffalo spermatozoa. Buffalo Bulletin, 40(1), 115–121. Retrieved from https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/633
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