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A male, 3 years old nondescript buffalo calf was presented with the complaint of epiphora, blepherospasm, pigmentation and impaired vision of the affected left eye. Clinical findings was similar as history with inflammatory signs on cornea and conjunctiva. Microscopic examination confirmed that the animal was affected with Staphylococcus areus. Based on history, clinical findings and microscopic examination the condition was diagnosed as Staphylococcus areus infected keratoconjunctivitis. For perfect treatment, antibiotic sensitivity test was performed and Gentamicin found more effective against infection. The animal was treated topically with combination of Gentamicin and Dexamethasone eye drop 4 drop on affected eye. Parentrally, Inj. Oxytetracycline 10 mg/kg body weight and combination of Vitamin A, D, E as a supportive drug given intramuscularly. Animal was completely recovered on ninth day of follow up period.