Postbiotics: Next-gen bioactives for functional food innovation

Main Article Content

Kanthida Wadeesirisak

Abstract

In recent years, postbiotics have attracted significant attention in the fields of food and nutrition as bioactive compounds produced by beneficial microbes, independent of live microorganisms. Postbiotics consist of various substances, including short-chain fatty acids, antimicrobial peptides, enzymes, polysaccharides, and inactivated microbial cells. These compounds play important roles in promoting gut health, boosting immunity, and potentially improving protein absorption. Due to these functional properties, the food industry has begun incorporating postbiotics into various product categories such as fermented foods, plant-based dairy alternatives, and dietary supplements. Compared to prebiotics and probiotics, postbiotics offer notable advantages in terms of safety and biological stability in certain applications. This article aims to present knowledge on the types, mechanisms of action, benefits, and trends in the application of postbiotics in the food industry, providing insights for the development of future functional food products.

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References

Kumar A, Green KM, Rawat M. A comprehensive overview of postbiotics with a special focus on discovery techniques and clinical applications. Foods. 2024;13(18):2937. DOI: https://doi.org/10.3390/foods13182937

Salminen S, Collado MC, Endo A, Hill C, Lebeer S, Quigley EMM, et al. The international scientific association of probiotics and prebiotics (ISAPP) consensus statement on the definition and scope of postbiotics. Nature Reviews Gastroenterology & Hepatology. 2021;18(9):649-67. DOI: https://doi.org/10.1038/s41575-021-00440-6

Aguilar-Toalá JE, Garcia-Varela R, Garcia HS, Mata-Haro V, González-Córdova AF, Vallejo-Cordoba B, et al. Postbiotics: An evolving term within the functional foods field. Trends in Food Science & Technology. 2018;75:105-14. DOI: https://doi.org/10.1016/j.tifs.2018.03.009

Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, et al. The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology & Hepatology. 2014;11(8):506-14. DOI: https://doi.org/10.1038/nrgastro.2014.66

Fiore W, Arioli S, Guglielmetti S. The neglected microbial components of commercial probiotic formulations. Microorganisms. 2020;8(8). DOI: https://doi.org/10.3390/microorganisms8081177

Asif A, Afzaal M, Shahid H, Saeed F, Ahmed A, Shah YA, et al. Probing the functional and therapeutic properties of postbiotics in relation to their industrial application. Food Sci Nutr. 2023;11(8):4472-84. DOI: https://doi.org/10.1002/fsn3.3465

Ma L, Tu H, Chen T. Postbiotics in Human Health: A Narrative Review. Nutrients. 2023;15(2).

Wu MH, Pan TM, Wu YJ, Chang SJ, Chang MS, Hu CY. Exopolysaccharide activities from probiotic Bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties. Int J Food Microbiol. 2010;144(1):104-10. DOI: https://doi.org/10.1016/j.ijfoodmicro.2010.09.003

Foong O, Foo HL, Loh T, Mohamad R, Rahim R, Ariff A. A refined medium to enhance the antimicrobial activity of postbiotic produced by Lactiplantibacillus plantarum RS5. Scientific Reports. 2021;11. DOI: https://doi.org/10.1038/s41598-021-87081-6

Benítez-Chao DF, León-Buitimea A, Lerma-Escalera JA, Morones-Ramírez JR. Bacteriocins: An overview of antimicrobial, toxicity, and biosafety assessment by in vivo models. Front Microbiol. 2021;12:630695. DOI: https://doi.org/10.3389/fmicb.2021.630695

Tong Y, Abbas Z, Zhang J, Wang J, Zhou Y, Si D, et al. Antimicrobial activity and mechanism of novel postbiotics against foodborne pathogens. LWT. 2025;217:117464. DOI: https://doi.org/10.1016/j.lwt.2025.117464

Asadi Z, Abbasi A, Ghaemi A, Montazeri EA, Akrami S. Investigating the properties and antibacterial, antioxidant, and cytotoxicity activity of postbiotics derived from Lacticaseibacillus casei on various gastrointestinal pathogens in vitro and in food models. GMS Hyg Infect Control. 2024;19:Doc60.

Davarzani S, Sanjabi MR, Mojgani N, Mirdamadi S, Soltani M. Investigating the antibacterial, antioxidant, and cholesterol-lowering properties of yogurt fortified with postbiotic of Lactobacillus acidophilus and Lactiplantibacillus plantarum in the wistar rat model. Journal of Food Protection. 2024;87(12):100408. DOI: https://doi.org/10.1016/j.jfp.2024.100408

Vera-Santander VE, Mani-López E, López-Malo A, Jiménez-Munguía MT. Use of whey for a sustainable production of postbiotics with potential bioactive metabolites. Sustainable Food Technology. 2024;2(4):1101-12. DOI: https://doi.org/10.1039/D4FB00061G

Guo C, Huo YJ, Li Y, Han Y, Zhou D. Gut-brain axis: Focus on gut metabolites short-chain fatty acids. World J Clin Cases. 2022;10(6):1754-63. DOI: https://doi.org/10.12998/wjcc.v10.i6.1754

Thorakkattu P, Khanashyam AC, Shah K, Babu KS, Mundanat AS, Deliephan A, et al. Postbiotics: current trends in food and pharmaceutical industry. Foods. 2022;11(19). DOI: https://doi.org/10.3390/foods11193094

Behare P, Singh R, Singh RP. Exopolysaccharide-producing mesophilic lactic cultures for preparation of fat-free Dahi - an Indian fermented milk. J Dairy Res. 2009;76(1):90-7. DOI: https://doi.org/10.1017/S0022029908003865

Ahmad Rather I, Seo BJ, Rejish Kumar VJ, Choi UH, Choi KH, Lim JH, et al. Isolation and characterization of a proteinaceous antifungal compound from Lactobacillus plantarum YML007 and its application as a food preservative. Lett Appl Microbiol. 2013;57(1):69-76. DOI: https://doi.org/10.1111/lam.12077

Makhal S, Kanawjia SK, Giri A. Effect of microGARD on keeping quality of direct acidified Cottage cheese. J Food Sci Technol. 2015;52(2):936-43. DOI: https://doi.org/10.1007/s13197-013-1055-2

Masiá C, Geppel A, Jensen PE, Buldo P. Effect of Lactobacillus rhamnosus on physicochemical properties of fermented plant-based raw materials. Foods. 2021;10(3). DOI: https://doi.org/10.3390/foods10030573

Wegh CAM, Geerlings SY, Knol J, Roeselers G, Belzer C. Postbiotics and their potential applications in early life nutrition and beyond. International journal of molecular sciences. 2019; 20(19):4673. DOI: https://doi.org/10.3390/ijms20194673

Ma L, Tu H, Chen T. Postbiotics in human health: A narrative review. Nutrients. 2023;15(2):291. DOI: https://doi.org/10.3390/nu15020291

Islam F, Azmat F, Imran A, Zippi M, Hong W, Tariq F, et al. Role of postbiotics in food and health: a comprehensive review. CyTA - Journal of Food. 2024;22(1):2386412. DOI: https://doi.org/10.1080/19476337.2024.2386412

Barros CP, Guimarães JT, Esmerino EA, Duarte MCKH, Silva MC, Silva R, et al. Paraprobiotics and postbiotics: concepts and potential applications in dairy products. Current Opinion in Food Science. 2020;32:1-8. DOI: https://doi.org/10.1016/j.cofs.2019.12.003

Bueno EBT, Silva KdO, Mendes MEF, de Oliveira LB, Menezes FPd, Imperador AC, et al. Postbiotics derived from lactic acid bacteria fermentation: Therapeutic potential in the treatment of muscular complications in inflammatory bowel disease. Fermentation. 2025;11(7):362. DOI: https://doi.org/10.3390/fermentation11070362

Taverniti V, Guglielmetti S. The immunomodulatory properties of probiotic microorganisms beyond their viability (ghost probiotics: proposal of paraprobiotic concept). Genes Nutr. 2011;6(3):261-74. DOI: https://doi.org/10.1007/s12263-011-0218-x

Żółkiewicz J, Marzec A, Ruszczyński M, Feleszko W. Postbiotics-a step beyond pre- and probiotics. Nutrients. 2020;12(8). DOI: https://doi.org/10.3390/nu12082189

Anbalagan C, Nandabalan SK, Sankar P, Rajaram PS, Govindaraj K, Rupert S, et al. Postbiotics of naturally fermented synbiotic mixture of rice water aids in promoting colonocyte health. Biomolecules. 2024;14(3):344. DOI: https://doi.org/10.3390/biom14030344

Muchimapura S, Thukhammee W, Phuthong S, Potue P, Khamseekaew J, Tong-un T, et al. Mung bean functional protein enhances endothelial function via antioxidant activity and inflammation modulation in middle-aged adults: a randomized double-blind trial. Foods. 2024;13(21):3427. DOI: https://doi.org/10.3390/foods13213427

Postbiotics Market to Reach $32.73 Million by 2032, Growing at a CAGR of 10.4% from 2025--Exclusive Report by Meticulous Research®. PR Newswire [Internet]. 2025 April [cited 2025 May 12]. Available from: https://finance.yahoo.com/news/postbiotics-market-reach-32-73-140100298.html?utm.

Prajapati N, Patel J, Singh S, Yadav VK, Joshi C, Patani A, et al. Postbiotic production: harnessing the power of microbial metabolites for health applications. Front Microbiol. 2023;14:1306192. DOI: https://doi.org/10.3389/fmicb.2023.1306192