Preliminary study on protein hydrolysates from Spirulina using enzymatic hydrolysis
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Abstract
Spirulina is classified as microalgae and is composed of high quality protein. It contains trace amounts of essential amino acids and bioactive peptides, including important biological active substances such as phenolic compounds. Therefore, it helps to promote the consumer’s health. Protein hydrolysates are a source of a variety of bioactive peptides. Protein hydrolysates were obtained from Spirulina biomass using various conditions of enzymatic hydrolysis. The results found that the concentration of protein hydrolyses with alkaline protease was 6.33 mg/ml. However, hydrolysis with alcalase enzyme (Alcalase®) resulted in the highest protein concentration of 0.58 mg/ml. Total phenolic contents obtained from alcalase hydrolysis were greater than that of alkaline protease enzyme. Although different types of enzymes showed an effect on hydrolysis yield, protein yields were similar when the enzyme concentration and the hydrolysis time were varied.
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