BLOOD PROFILE OF ZINC AND COPPER AT ESTRUS STAGE AND ITS EFFECT ON SUBSEQUENT PREGNANCIES IN BUFFALOES

Mudassar Saleem; Atique Shazad; Mudussar Nawaz; Asghar Khan; Ejaz Ahmad; Zahid Naseer

doi:10.56825/bufbu.2025.4445527

Authors

Mudassar Saleem
Atique Shazad
Mudussar Nawaz
Asghar Khan
Ejaz Ahmad
Zahid Naseer Pir Mehr Ali Shah arid agriculture university 1

DOI:

https://doi.org/10.56825/bufbu.2025.4445527

Keywords:

Bubalus bubalis, buffaloes, Cu, Zn, estrus, pregnancy

Abstract

The present study aimed to determine the blood profile of copper (Cu) and zinc (Zn) concentrations at estrus and subsequent pregnancy in Nili-Ravi buffaloes. Fifty-one estrus buffaloes (4 to 6 years of age) were selected based on physical signs and rectal palpation and were inseminated on an am-pm basis. The blood samples were collected at the time of artificial insemination (AI) via jugular venipuncture from 51 buffaloes, and 21 buffaloes were further bled at day 16 of AI and pregnancy diagnosis time. The concentrations of Cu and Zn in blood sera were determined using an automatic chemistry analyser. The pregnancy diagnosis was performed through rectal palpation at day sixty post-AI. The pregnancy confirmation was based on the asymmetry of uterine horns with fetal fluids. The results indicate that Zn levels were higher numerically in pregnant than nonpregnant buffaloes. The serum Cu concentration was also similar between pregnant and nonpregnant buffaloes. The conception rate in buffaloes with a standard Zn level was significantly higher than in Zn-deficient buffaloes. There was an increasing tendency of conception in the buffaloes with a standard concentration of Cu as compared to Cu-deficient buffaloes. Post-AI changes in Zn concentration were negligible, but the concentration of Cu decreased with the advancement in pregnancy. In conclusion, a higher Zn level at the time of AI successfully established pregnancy in buffaloes.

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